Formalin-fixed, paraffin-embedded human Tonsil stained with G-CSF Monoclonal Antibody (CSF3/9). Note specific cytoplasmic staining of granulocytes.
Formalin-fixed, paraffin-embedded human Tonsil stained with G-CSF Monoclonal Antibody (CSF3/9). Note specific cytoplasmic staining of granulocytes.

Anti-Granulocyte-Colony Stimulating Factor Antibody Clone CSF3/900

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: 1440-MSM1
Species: Human and Macaque Monkey
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 1440-MSM1-P1
Granulocyte-Colony Stimulating Factor General Information
Alternate Names
Insulin-like growth factor-binding protein 1, IBP-1, placental protein 12, PP12, IGFBP1
Molecular Weight
19kDa
Chromosomal Location
17q21.1
Curated Database and Bioinformatic Data
Gene SymbolCSF3
Entrez Gene ID1440
Ensemble Gene IDENSG00000108342
RefSeq Protein Accession(s)NP_757373, NP_757374, NP_000750, NP_001171618
RefSeq mRNA Accession(s)NM_000759, NM_172220, NM_001178147, NM_172219, NR_033662
RefSeq Genomic Accession(s)NC_018928, NC_000017
UniProt ID(s)P09919, Q8N4W3, Q6FH65
UniGene ID(s)P09919, Q8N4W3, Q6FH65
HGNC ID(s)2438
Cosmic ID(s)CSF3
KEGG Gene ID(s)hsa:1440
PharmGKB ID(s)PA26941
General Description of Granulocyte-Colony Stimulating Factor.
This MAb recognizes granulocyte-colony stimulating factor (G-CSF) in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell types. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. It reacts with early precursor, mature forms of myeloid cells. It is useful for the detection of myeloid leukemias, granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes.G-CSF is a pleiotropic cytokine that influences differentiation, proliferation, activation of the neutrophilic granulocyte lineage. The human G-CSF cDNA encodes a 207 amino acid precursor containing a 29 amino acid signal peptide that is proteolytically cleaved to form a 178 amino acid residue mature protein. Two G-CSFs, which are identical except for a three amino acid deletion in the amino-terminus of one form of the protein have been isolated from human cells. Murine, human G-CSFs share 73% sequence identity at the amino acid level.

Human and Macaque Monkey Anti-Granulocyte-Colony Stimulating Factor Antibody Product Attributes

Species: Human and Macaque Monkey
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Granulocyte-Colony Stimulating Factor Antibody Clone: CSF3/900
Clone CSF3/900 Host and Isotype: Mouse IgG1
Anti-Human and Macaque Monkey Granulocyte-Colony Stimulating Factor Positive Control Sample: HL60 cells. Tonsil or lymph node
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Human recombinant full-length CSF3 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Granulocyte-Colony Stimulating Factor Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in Granulocyte-Colony Stimulating Factor antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in exocrine glandular cells in the pancreas. More moderate antibody staining intensity was present in exocrine glandular cells in the pancreas. Low, but measureable presence of Granulocyte-Colony Stimulating Factor could be seen in cells in the endometrial stroma in endometrium, cells in the molecular layer in cerebellum, decidual cells in the placenta, germinal center cells in the tonsil, glandular cells in the adrenal gland, appendix, breast, gallbladder, parathyroid gland, prostate, rectum and thyroid gland, hematopoietic cells in the bone marrow, keratinocytes in skin, Langerhans in skin, Leydig cells in the testis, lymphoid tissue in appendix, macrophages in lung, neuronal cells in the caudate nucleus, cerebral cortex and hippocampus, ovarian stroma cells in the ovary, peripheral nerve/ganglion in colon, Purkinje cells in the cerebellum, respiratory epithelial cells in the bronchus and nasopharynx, squamous epithelial cells in the tonsil, trophoblastic cells in the placenta and urothelial cells in the urinary bladder. We were unable to detect Granulocyte-Colony Stimulating Factor in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Granulocyte-Colony Stimulating Factor expression as measured by anti-Granulocyte-Colony Stimulating Factor antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity ++ ++ - + - - - + - - - - + + ++ - + - - +
CSF3 Variability + ++ ++ ++ ++ ++ + ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++

Limitations and Warranty

enQuire Bio's Granulocyte-Colony Stimulating Factor Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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