Human, Monkey, Cow, Pig, Mouse, and Fish Anti-Heparan Sulfate Proteoglycan / Perlecan Antibody Product Attributes
Species: Human, Monkey, Cow, Pig, Mouse, and Fish
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (1-2ug of antibody/ml for 30 minutes at RT)
Anti-Heparan Sulfate Proteoglycan / Perlecan Antibody Clone: A7L6
Clone A7L6 Host and Isotype: Rat IgG2a kappa
Anti-Human, Monkey, Cow, Pig, Mouse, and Fish Heparan Sulfate Proteoglycan / Perlecan Positive Control Sample: Breast carcinomas, squamous cell carcinomas.
Cellular Localization of Antibody
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Murine EHS laminin preparation
Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.
Heparan Sulfate Proteoglycan / Perlecan Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:Anti-HSPG2 antibody staining is expected to be primarily localized to the cytosol, nucleoplasm and plasma membrane.
Observed Antibody Staining Data By Tissue Type:Variations in Heparan Sulfate Proteoglycan / Perlecan antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of Heparan Sulfate Proteoglycan / Perlecan could be seen inadipocytes in mesenchymal tissue, cells in the tubules in kidney, decidual cells in the placenta, endothelial cells in the cerebral cortex, epidermal cells in the skin, exocrine glandular cells in the pancreas, fibroblasts in skin, germinal center cells in the tonsil, glandular cells in the appendix, breast, cervix, uterine, colon, gallbladder, parathyroid gland, rectum and stomach, hematopoietic cells in the bone marrow, hepatocytes in liver, islets of Langerhans in pancreas, Leydig cells in the testis, lymphoid tissue in appendix, macrophages in lung, melanocytes in skin, myoepithelial cells in the breast, non-germinal center cells in the lymph node and tonsil, ovarian stroma cells in the ovary, respiratory epithelial cells in the bronchus, smooth muscle cells in the smooth muscle, squamous epithelial cells in the oral mucosa and tonsil and urothelial cells in the urinary bladder. We were unable to detect Heparan Sulfate Proteoglycan / Perlecan in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Observed Antibody Staining Data By Tissue Disease Status:Tissues from cancer patients, for instance, have their own distinct pattern of Heparan Sulfate Proteoglycan / Perlecan expression as measured by anti-Heparan Sulfate Proteoglycan / Perlecan antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
|Sample Type||breast cancer||carcinoid||cervical cancer||colorectal cancer||endometrial cancer||glioma||head and neck cancer||liver cancer||lung cancer||lymphoma||melanoma||ovarian cancer||pancreatic cancer||prostate cancer||renal cancer||skin cancer||stomach cancer||testicular cancer||thyroid cancer||urothelial cancer|
Limitations and Warranty
enQuire Bio's Heparan Sulfate Proteoglycan / Perlecan Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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