Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Heparan Sulfate Monoclonal Antibody (SPM255).
Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Heparan Sulfate Monoclonal Antibody (SPM255).

Anti-Heparan Sulfate Proteoglycan / Perlecan Antibody Clone SPM255

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: 3339-RTM1X
Species: Human, Monkey, Cow, Pig, Mouse, and Fish
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Rat IgG2a kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 3339-RTM1X-P1
Heparan Sulfate Proteoglycan / Perlecan General Information
Alternate Names
Perlecan, Basement membrane-specific heparan sulfate proteoglycan core protein, HSPG, heparan sulfate proteoglycan 2, HSPG2
Molecular Weight
>400kDa
Chromosomal Location
1p36.1-p34
Curated Database and Bioinformatic Data
Gene SymbolHSPG2
Entrez Gene ID3339
Ensemble Gene IDENSG00000142798
RefSeq Protein Accession(s)XP_016856611, NP_001278789, XP_011539620, XP_016856609, NP_005520, XP_016856610
RefSeq mRNA Accession(s)XM_017001120, NM_005529, NM_001291860, XM_017001121, XM_017001122, XM_011541318,
RefSeq Genomic Accession(s)NC_018912, NC_000001, NG_016740
UniProt ID(s)P98160
UniGene ID(s)P98160
HGNC ID(s)5273
Cosmic ID(s)HSPG2
KEGG Gene ID(s)hsa:3339
PharmGKB ID(s)PA29537
General Description of Heparan Sulfate Proteoglycan / Perlecan.
This MAb specifically precipitates heterogeneous material of high MW, identified as perlecan, a major heparan-sulfate proteoglycan (HSPG) within all basement membranes, cell surfaces. It does not cross-react with laminin, fibronectin, or dermatran sulfate proteoglycan. Because of perlecan s strategic location, ability to store, protect growth factors, it has been strongly implicated in the control of tumor cell growth, metastatic behavior. Perlecan possesses angiogenic, growth-promoting attributes primarily by acting as a co-receptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth, neovascularization. Thus, perlecan is a potent inducer of neoplasm growth, angiogenesis in vivo, therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.

Human, Monkey, Cow, Pig, Mouse, and Fish Anti-Heparan Sulfate Proteoglycan / Perlecan Antibody Product Attributes

Species: Human, Monkey, Cow, Pig, Mouse, and Fish
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (1-2ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Heparan Sulfate Proteoglycan / Perlecan Antibody Clone: SPM255
Clone SPM255 Host and Isotype: Rat IgG2a kappa
Anti-Human, Monkey, Cow, Pig, Mouse, and Fish Heparan Sulfate Proteoglycan / Perlecan Positive Control Sample: Breast carcinomas, squamous cell carcinomas.
Cellular Localization of Antibody Basement membrane
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Murine EHS laminin preparation
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Heparan Sulfate Proteoglycan / Perlecan Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-HSPG2 antibody staining is expected to be primarily localized to the cytosol, nucleoplasm and plasma membrane.

Observed Antibody Staining Data By Tissue Type:

Variations in Heparan Sulfate Proteoglycan / Perlecan antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of Heparan Sulfate Proteoglycan / Perlecan could be seen inadipocytes in mesenchymal tissue, cells in the tubules in kidney, decidual cells in the placenta, endothelial cells in the cerebral cortex, epidermal cells in the skin, exocrine glandular cells in the pancreas, fibroblasts in skin, germinal center cells in the tonsil, glandular cells in the appendix, breast, cervix, uterine, colon, gallbladder, parathyroid gland, rectum and stomach, hematopoietic cells in the bone marrow, hepatocytes in liver, islets of Langerhans in pancreas, Leydig cells in the testis, lymphoid tissue in appendix, macrophages in lung, melanocytes in skin, myoepithelial cells in the breast, non-germinal center cells in the lymph node and tonsil, ovarian stroma cells in the ovary, respiratory epithelial cells in the bronchus, smooth muscle cells in the smooth muscle, squamous epithelial cells in the oral mucosa and tonsil and urothelial cells in the urinary bladder. We were unable to detect Heparan Sulfate Proteoglycan / Perlecan in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Heparan Sulfate Proteoglycan / Perlecan expression as measured by anti-Heparan Sulfate Proteoglycan / Perlecan antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity ++ + ++ ++ ++ + ++ + ++ ++ ++ + ++ + ++ ++ + ++ ++ ++
HSPG2 Variability ++ ++ ++ + ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ +

Limitations and Warranty

enQuire Bio's Heparan Sulfate Proteoglycan / Perlecan Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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