Human Anti-HER2 / ErbB2 / neu / CD340 Antibody Product Attributes
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed ) (0.5-1ug of antibody/ml for 30 min at RT)
Clonality: Monoclonal
Anti-HER2 / ErbB2 / neu / CD340 Antibody Clone: ERB2/776
Clone ERB2/776 Host and Isotype: Mouse IgG1 kappa
Anti-Human HER2 / ErbB2 / neu / CD340 Positive Control Sample: SKBR-3 Cells or Breast Cancers.
Cellular Localization of Antibody Cell Surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant intracellular domain of human HER2 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
HER2 / ErbB2 / neu / CD340 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-ERBB2 antibody staining is expected to be primarily localized to the cytosol and plasma membrane.Observed Antibody Staining Data By Tissue Type:
Variations in HER2 / ErbB2 / neu / CD340 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of HER2 / ErbB2 / neu / CD340 could be seen inglandular cells in the fallopian tube, parathyroid gland, prostate, rectum, salivary gland, seminal vesicle, small intestine and thyroid gland, hematopoietic cells in the bone marrow, hepatocytes in liver, melanocytes in skin, pneumocytes in lung, squamous epithelial cells in the cervix, uterine, esophagus, tonsil and vagina and glandular cells in the fallopian tube, parathyroid gland, prostate, rectum, salivary gland, seminal vesicle and small intestine and thyroid gland. We were unable to detect HER2 / ErbB2 / neu / CD340 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of HER2 / ErbB2 / neu / CD340 expression as measured by anti-HER2 / ErbB2 / neu / CD340 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.| Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal Intensity | ++ | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - | + |
| ERBB2 Variability | +++ | + | + | ++ | ++ | + | ++ | ++ | + | + | + | ++ | ++ | + | + | ++ | + | ++ | + | ++ |
Limitations and Warranty
enQuire Bio's HER2 / ErbB2 / neu / CD340 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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