Human, Mouse, and Rat Anti-Histone H1 Antibody Product Attributes
Species: Human, Mouse, and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Anti-Histone H1 Antibody Clone: AE-4
Clone AE-4 Host and Isotype: Mouse IgG2a kappa
Anti-Human, Mouse, and Rat Histone H1 Positive Control Sample: HeLa, A-431, LNCap or Jurkat cells. Breast carcinoma.
Cellular Localization of Antibody Nuclear
Buffer and Stabilizer: 1mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Nuclei of human leukemia biopsy cells
Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.
Histone H1 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-H1F0 antibody staining is expected to be primarily localized to the nuclear bodies and nucleus. There is variability in either the signal strength or the localization of signal in nucleus from cell to cell.
Limitations and Warranty
enQuire Bio's Histone H1 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.