Human Anti-HLA-DR Antibody Product Attributes
Cross Reactivity: baboon, chimpanzee, cynomolgus monkey, rhesus macaque
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1x105 to 1x108 cells.
Clonality: Monoclonal Antibody
Anti-HLA-DR Antibody Clone: L243
Clone L243 Host and Isotype: Mouse IgG2a kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
HLA-DR Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:Variations in HLA-DR antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the glomeruli in kidney, germinal center cells in the lymph node and tonsil, glandular cells in the duodenum and endometrium, Langerhans in skin, lymphoid tissue in appendix, macrophages in lung, myoepithelial cells in the breast and non-germinal center cells in the lymph node and tonsil. More moderate antibody staining intensity was present in cells in the glomeruli in kidney, germinal center cells in the lymph node and tonsil, glandular cells in the duodenum and endometrium, Langerhans in skin, lymphoid tissue in appendix, macrophages in lung, myoepithelial cells in the breast and non-germinal center cells in the lymph node and tonsil. Low, but measureable presence of HLA-DR could be seen in cells in the red pulp in spleen, cells in the tubules in kidney, endothelial cells in the colon, epidermal cells in the skin, glandular cells in the appendix, cervix, uterine, epididymis and fallopian tube, glial cells in the caudate nucleus and hippocampus, myocytes in skeletal muscle, peripheral nerve in mesenchymal tissue, squamous epithelial cells in the tonsil and vagina and urothelial cells in the urinary bladder. We were unable to detect HLA-DR in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Brodsky FM. 1984. Immunogenetics. 19(3): 179-194. Robbins PA, Evans EL, Ding AH, Warner NL and Brodsky FM. 1987. Human Immunol. 18(4): 301-313. Poulin LF, Reyal Y, Uronen-Hansson H, Schraml BU, Sancho D, Murphy KM, Hakansson UK, Moita LF, Agace WW, Bonnet D and Reis e Sousa C. 2012. Blood. 119(25): 6052-6062. (Flow cytometry) Shio MT, Hassan GS, Shah WA, Nadiri A, El Fakhry Y, Li H and Mourad W. 2014. J Immunol. 192(6): 2543-2550. (Flow cytometry) Bigley V, McGovern N, Milne P, Dickinson R, Pagan S, Cookson S, Haniffa M and Collin M. 2015. J Leukoc Biol. 97(4): 627-634. (Flow cytometry) Goodier MR and Londei M. 2000. J Immunol. 165(1): 139-147. (Depletion) Kalka-Moll WM, Tzianabos AO, Bryant PW, Niemeyer M, Ploegh HL and Kasper DL. 2002. J Immunol. 169(11): 6149-6153. (Blocking)
Limitations and Warranty
enQuire Bio's Human Anti-HLA-DR Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.