Formalin-fixed, paraffin-embedded human Tonsil stained with HLA-DRB Monoclonal Antibody (SPM288).
Formalin-fixed, paraffin-embedded human Tonsil stained with HLA-DRB Monoclonal Antibody (SPM288).

Anti-HLA-DRB Antibody Clone SPM288

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: 3123-MSM1XX
Species: Human, Monkey, and Mouse (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2b kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 3123-MSM1XX-P1
HLA-DRB General Information
Alternate Names
HLA class II histocompatibility antigen, DRB1 beta chain, HLA-DRB1
Molecular Weight
28kDa (beta chain)
Chromosomal Location
6p21.3
Curated Database and Bioinformatic Data
Gene SymbolHLA-DRB1
Entrez Gene ID3123
Ensemble Gene IDENSG00000206240, ENSG00000236884, ENSG00000206306, ENSG00000228080, ENSG00000229074, ENSG00000196126
RefSeq Protein Accession(s)XP_011546040, NP_001230894, NP_002115
RefSeq mRNA Accession(s)NM_001243965, XM_011547738, NM_002124
RefSeq Genomic Accession(s)NG_029921, NG_002392, NG_167248, NC_018917, NG_002433, NC_000006, NG_002432, NG_113891, NG_167245, NG_167249, NG_167246
UniProt ID(s)Q29974, D7RIG5, P01912, X5DNQ0, D7RIH8, Q5Y7D1, P13761, P01911
UniGene ID(s)Q29974, D7RIG5, P01912, X5DNQ0, D7RIH8, Q5Y7D1, P13761, P01911
HGNC ID(s)4948
Cosmic ID(s)HLA-DRB1
KEGG Gene ID(s)hsa:3123
PharmGKB ID(s)PA35072
General Description of HLA-DRB.
This MAb reacts with a 28kDa chain of HLA-DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP, HLA-DQ. The L243 antibody recognizes a different epitope than the LN3 monoclonal antibody,, these antibodies do not cross-block binding to each other's respective epitopes. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain, a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells, other non-professional APCs. In conjunction with the CD3/TCR complex, CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells, their corresponding neoplastic counterparts. Loss of HLA-DR expression is related to tumor microenvironment, predicts adverse outcome in diffuse large B-cell lymphoma.

Human, Monkey, and Mouse (-) Anti-HLA-DRB Antibody Product Attributes

Species: Human, Monkey, and Mouse (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.25-0.5ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-HLA-DRB Antibody Clone: SPM288
Clone SPM288 Host and Isotype: Mouse IgG2b kappa
Anti-Human, Monkey, and Mouse (-) HLA-DRB Positive Control Sample: Ramos, Daudi or HuT78 cells. Tonsil or lymph node
Cellular Localization of Antibody Cell Surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Activated human peripheral blood mononuclear cells
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

HLA-DRB Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in HLA-DRB antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in germinal center cells in the lymph node and tonsil, macrophages in lung and non-germinal center cells in the lymph node and tonsil. More moderate antibody staining intensity was present in germinal center cells in the lymph node and tonsil, macrophages in lung and non-germinal center cells in the lymph node and tonsil. Low, but measureable presence of HLA-DRB could be seen in cells in the red pulp in spleen, cells in the white pulp in spleen and pneumocytes in lung. We were unable to detect HLA-DRB in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of HLA-DRB expression as measured by anti-HLA-DRB antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - - - - - ++ - - - - - - - - - -
HLA-DRB1 Variability + + + + + + + + ++ +++ ++ + + + ++ + ++ ++ ++ ++

Limitations and Warranty

enQuire Bio's HLA-DRB Anti-Human, Monkey Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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