Staining by anti-HSP27 Antibody 1

Anti-HSP27 Antibody Clone SPM252

$ 298.00
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SKU: 3315-MSM1X
Species: Human, Chimpanzee, Sheep, Rat, Mouse, and Chicken
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 3315-MSM1X-P1
HSP27 General Information
Alternate Names
Heat shock protein 27, Hsp27, heat shock protein beta-1, HSPB1, HSPB1 , Hsp 27
Molecular Weight
27kDa
Chromosomal Location
7q11.23
Curated Database and Bioinformatic Data
Gene SymbolHSPB1
Entrez Gene ID3315
Ensemble Gene IDENSG00000106211
RefSeq Protein Accession(s)NP_001531
RefSeq mRNA Accession(s)NM_001540,
RefSeq Genomic Accession(s)NG_008995, NC_018918, NC_000007
UniProt ID(s)V9HW43, P04792
UniGene ID(s)V9HW43, P04792
HGNC ID(s)5246
Cosmic ID(s)HSPB1
KEGG Gene ID(s)hsa:3315
PharmGKB ID(s)PA29511
General Description of HSP27.
It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced p29, 24K protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen,/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.

Human, Chimpanzee, Sheep, Rat, Mouse, and Chicken Anti-HSP27 Antibody Product Attributes

Species: Human, Chimpanzee, Sheep, Rat, Mouse, and Chicken
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Western Blot (0.25-0.5ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-HSP27 Antibody Clone: SPM252
Clone SPM252 Host and Isotype: Mouse IgG1 kappa
Anti-Human, Chimpanzee, Sheep, Rat, Mouse, and Chicken HSP27 Positive Control Sample: ~50% of Breast Carcinomas are positive for hsp27 especially those that are also positive for estrogen,/or progesterone receptor.
Cellular Localization of Antibody Predominantly cytoplasmic with some nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells.
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

HSP27 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-HSPB1 antibody staining is expected to be primarily localized to the cytosol and plasma membrane.

Observed Antibody Staining Data By Tissue Type:

Variations in HSP27 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in decidual cells in the placenta, endothelial cells in the colon, epidermal cells in the skin, exocrine glandular cells in the pancreas, follicle cells in the ovary, glandular cells in the endometrium, epididymis, fallopian tube and gallbladder, keratinocytes in skin, Langerhans in skin, melanocytes in skin, myocytes in heart muscle, respiratory epithelial cells in the bronchus and nasopharynx, squamous epithelial cells in the esophagus, oral mucosa, tonsil and vagina, trophoblastic cells in the placenta and urothelial cells in the urinary bladder. More moderate antibody staining intensity was present in decidual cells in the placenta, endothelial cells in the colon, epidermal cells in the skin, exocrine glandular cells in the pancreas, follicle cells in the ovary, glandular cells in the endometrium, epididymis, fallopian tube and gallbladder, keratinocytes in skin, Langerhans in skin, melanocytes in skin, myocytes in heart muscle, respiratory epithelial cells in the bronchus and nasopharynx, squamous epithelial cells in the esophagus, oral mucosa, tonsil and vagina, trophoblastic cells in the placenta and urothelial cells in the urinary bladder. Low, but measureable presence of HSP27 could be seen inadipocytes in mesenchymal tissue, bile duct cells in the liver, fibroblasts in mesenchymal tissue, glandular cells in the breast and colon, hematopoietic cells in the bone marrow, hepatocytes in liver, islets of Langerhans in pancreas, Leydig cells in the testis and pneumocytes in lung. We were unable to detect HSP27 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of HSP27 expression as measured by anti-HSP27 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity ++ - + - - - + - ++ - ++ ++ ++ - ++ ++ - - + ++
HSPB1 Variability ++ + +++ ++ ++ + ++ ++ ++ + + ++ ++ + ++ ++ ++ + ++ +++

Limitations and Warranty

enQuire Bio's HSP27 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-HSP27 Antibody Clone SPM252