Formalin-paraffin human Prostate Carcinoma stained with IDH1 MAb (IDH1/1152).
Formalin-paraffin human Prostate Carcinoma stained with IDH1 MAb (IDH1/1152).

Anti-IDH1 Antibody Clone IDH1/1152

$ 298.00
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SKU: 3417-MSM2
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 3417-MSM2-P1
IDH1 General Information
Alternate Names
soluble Isocitrate dehydrogenase 1, soluble NADP+, IDH1
Molecular Weight
45-47kDa
Chromosomal Location
2q33.3
Curated Database and Bioinformatic Data
Gene SymbolIDH1
Entrez Gene ID3417
Ensemble Gene IDENSG00000138413
RefSeq Protein Accession(s)NP_001269316, NP_005887, NP_001269315
RefSeq mRNA Accession(s)NM_001282386, NM_001282387 NM_005896
RefSeq Genomic Accession(s)NC_018913, NC_000002, NG_023319
UniProt ID(s)V9HWJ2, O75874
UniGene ID(s)V9HWJ2, O75874
HGNC ID(s)5382
Cosmic ID(s)IDH1
KEGG Gene ID(s)hsa:3417
PharmGKB ID(s)PA29630
General Description of IDH1.
It recognizes a 45kDa protein, which is identified as isocitrate dehydrogenase (IDH1). It belongs to the isocitrate, isopropylmalate dehydrogenases family. IDH1 catalyzes the third step of the citric acid cycle, which involves the oxidative decarboxylation of isocitrate, forming ? -ketoglutarate, CO2 in a two-step reaction. The first step involves the oxidation of isocitrate to the intermediate oxalosuccinate, while the second step involves the production of ? -ketoglutarate. During this process, either NADH or NADPH is produced along with CO2. Recently, an inactivating mutation of IDH1 has been implicated in glioblastoma. IDH1 appears to function as a tumor suppressor that, when mutationally inactivated, contributes to tumorigenesis in part through induction of the HIF-1 pathway.

Human Anti-IDH1 Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-IDH1 Antibody Clone: IDH1/1152
Clone IDH1/1152 Host and Isotype: Mouse IgG1 kappa
Anti-Human IDH1 Positive Control Sample: HepG2, HeLa, HT29 or MCF7 cells. Breast, Colon or Prostate Carcinoma.
Cellular Localization of Antibody Cytoplasmic & Nuclear
Buffer and Stabilizer: 1mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant fragment (119 Amino acid residues around aa 280-420) of human IDH1 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

IDH1 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-idh1 antibody staining is expected to be primarily localized to the cytosol and nuclear bodies. There is variability in either the signal strength or the localization of signal in nuclear bodies from cell to cell.

Observed Antibody Staining Data By Tissue Type:

Variations in IDH1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the seminiferous ducts in testis, chondrocytes in mesenchymal tissue, glandular cells in the adrenal gland, epididymis, gallbladder, prostate and seminal vesicle, Leydig cells in the testis and urothelial cells in the urinary bladder. More moderate antibody staining intensity was present in cells in the seminiferous ducts in testis, chondrocytes in mesenchymal tissue, glandular cells in the adrenal gland, epididymis, gallbladder, prostate and seminal vesicle, Leydig cells in the testis and urothelial cells in the urinary bladder. Low, but measureable presence of IDH1 could be seen inadipocytes in breast and mesenchymal tissue, glandular cells in the cervix, uterine, colon, fallopian tube and salivary gland, macrophages in lung, myoepithelial cells in the breast, ovarian stroma cells in the ovary, pneumocytes in lung, respiratory epithelial cells in the bronchus and nasopharynx and squamous epithelial cells in the esophagus. We were unable to detect IDH1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of IDH1 expression as measured by anti-IDH1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - ++ - - + + - - - - - + + +++ - - + - - -
IDH1 Variability ++ ++ + ++ +++ ++ ++ ++ ++ + + +++ ++ ++ + + ++ ++ + ++

Limitations and Warranty

enQuire Bio's IDH1 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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