Human and Rat Anti-IgA Secretory Component / ECM1 Antibody Product Attributes
Species: Human and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-IgA Secretory Component / ECM1 Antibody Clone: SC05
Clone SC05 Host and Isotype: Mouse IgG1 kappa
Anti-Human and Rat IgA Secretory Component / ECM1 Positive Control Sample: Stomach, Lung, or breast tumor
Cellular Localization of Antibody Cell surface, cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Secretory Component protein isolated from human colostrum
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
IgA Secretory Component / ECM1 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-ECM1 antibody staining is expected to be primarily localized to the cytosol and nucleoplasm.Observed Antibody Staining Data By Tissue Type:
Variations in IgA Secretory Component / ECM1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glandular cells in the epididymis. More moderate antibody staining intensity was present in glandular cells in the epididymis. Low, but measureable presence of IgA Secretory Component / ECM1 could be seen inadipocytes in breast, chondrocytes in mesenchymal tissue, follicle cells in the ovary, glandular cells in the adrenal gland, cervix, uterine, seminal vesicle and thyroid gland, neuropil in cerebral cortex, peripheral nerve in mesenchymal tissue and respiratory epithelial cells in the bronchus and nasopharynx. We were unable to detect IgA Secretory Component / ECM1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of IgA Secretory Component / ECM1 expression as measured by anti-IgA Secretory Component / ECM1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Signal Intensity | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
ECM1 Variability | ++ | ++ | + | + | + | + | + | + | + | + | + | + | ++ | + | + | + | + | + | ++ | + |
Limitations and Warranty
enQuire Bio's IgA Secretory Component / ECM1 Anti-Human, Rat Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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