Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with IgA Secretory Component Monoclonal Antibody (SPM217).
Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with IgA Secretory Component Monoclonal Antibody (SPM217).

Anti-IgA Secretory Component / ECM1 Antibody Clone SPM217

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: 1893-MSM1X
Species: Human and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 1893-MSM1X-P1
IgA Secretory Component / ECM1 General Information
Alternate Names
Extracellular matrix protein 1, ECM1, EMC-1
Molecular Weight
~80kDa
Chromosomal Location
1q21.2
Curated Database and Bioinformatic Data
Gene SymbolECM1
Entrez Gene ID1893
Ensemble Gene IDENSG00000143369
RefSeq Protein Accession(s)NP_073155, NP_004416, NP_001189787
RefSeq mRNA Accession(s)NM_001202858, NM_022664, NM_004425
RefSeq Genomic Accession(s)NG_012062, NC_000001, NC_018912
UniProt ID(s)Q16610, A0A140VJI7
UniGene ID(s)Q16610, A0A140VJI7
HGNC ID(s)3153
Cosmic ID(s)ECM1
KEGG Gene ID(s)hsa:1893
PharmGKB ID(s)PA27598
General Description of IgA Secretory Component / ECM1.
This MAb reacts with a reduction-resistant epitope present in both free, SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution, level of both free, bound secretory component. Secretory component is differentially expressed in epithelium,, the antibody is a popular marker for identifying subpopulations of epithelial cells, epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation, tumorigenesis, all processes associated with differential secretory component expression.

Human and Rat Anti-IgA Secretory Component / ECM1 Antibody Product Attributes

Species: Human and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-IgA Secretory Component / ECM1 Antibody Clone: SPM217
Clone SPM217 Host and Isotype: Mouse IgG1 kappa
Anti-Human and Rat IgA Secretory Component / ECM1 Positive Control Sample: Stomach, Lung, or breast tumor
Cellular Localization of Antibody Cell surface, cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Secretory Component protein isolated from human colostrum
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

IgA Secretory Component / ECM1 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-ECM1 antibody staining is expected to be primarily localized to the cytosol and nucleoplasm.

Observed Antibody Staining Data By Tissue Type:

Variations in IgA Secretory Component / ECM1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glandular cells in the epididymis. More moderate antibody staining intensity was present in glandular cells in the epididymis. Low, but measureable presence of IgA Secretory Component / ECM1 could be seen inadipocytes in breast, chondrocytes in mesenchymal tissue, follicle cells in the ovary, glandular cells in the adrenal gland, cervix, uterine, seminal vesicle and thyroid gland, neuropil in cerebral cortex, peripheral nerve in mesenchymal tissue and respiratory epithelial cells in the bronchus and nasopharynx. We were unable to detect IgA Secretory Component / ECM1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of IgA Secretory Component / ECM1 expression as measured by anti-IgA Secretory Component / ECM1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - - - - - - - - - - - - - - - -
ECM1 Variability ++ ++ + + + + + + + + + + ++ + + + + + ++ +

Limitations and Warranty

enQuire Bio's IgA Secretory Component / ECM1 Anti-Human, Rat Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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