Mouse lymphoid cells were stimulated in the presence of a protein transport inhibitor. Cells were then fixed, permeabilized, stained with APC Mouse Anti-CD4 and intracellularly with 0.125 ug PE Mouse Anti-IL-4 (QAB81) (right panel) or 0.125 ug PE Rat Ig

Anti-IL-4 Antibody [11B11]

$ 69.00$ 259.00
Please Select Product Options Below To View The Catalog Number.

Species: Mouse
Tested Applications: Flow Cytometry
Available Conjugates: APC ;Unconjugated ;PE
Isotype: Rat IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
QAB81-APC-25ug Size: 25 ug, Tag: APC $ 69.00
QAB81-100ug Size: 100 ug, Tag: Unconjugated $ 89.00
QAB81-500ug Size: 500 ug, Tag: Unconjugated $ 179.00
QAB81-APC-100ug Size: 100 ug, Tag: APC $ 219.00
QAB81-PE-100ug Size: 100 ug, Tag: PE $ 209.00
QAB81-PE-25ug Size: 25 ug, Tag: PE $ 79.00
QAB81-QF630-100ug Size: 100 tests, Tag: Qfluor™ 630 $ 169.00
QAB81-QF630-500ug Size: 500 tests, Tag: Qfluor™ 630 $ 259.00
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In Stock.
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Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

Expiration Date:

Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

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Bioactivity (test results eg. IU/ml):

SKU: QAB81-APC-25ug
IL-4 / Interleukin-4 General Information
Alternate Names
IL4, Interleukin-4, BSF-1, BSF1, IL-4, BCGF1, BCGF-1
Curated Database and Bioinformatic Data
Gene SymbolIl4
Entrez Gene ID16189
Ensemble Gene IDENSMUSG00000000869
RefSeq Protein Accession(s)NP_067258
RefSeq mRNA Accession(s)NM_021283, NR_027491
RefSeq Genomic Accession(s)NC_000077
UniProt ID(s)P07750
UniGene ID(s)P07750
Cosmic ID(s)Il4
KEGG Gene ID(s)mmu:16189
General Description of IL-4 / Interleukin-4.
The 11B11 antibody binds to mouse Interleukin-4 (IL-4), a 14 kDa cytokine that is largely secreted by activated T cells of the Th2 subset, and to some degree by NKT and mast cells. This cytokine acts as a stimulatory factor for B cells, inducing their proliferation and differentiation, as well as playing a role in immunoglobulin class-switching. IL-4 may also provide autocrine stimulation for T cells, and affect the function of antigen presenting cells such as macrophages and dendritic cells. IL-4 can bind and signal via three cell surface receptor types: CD124 by itself, CD124 in combination with the common gamma chain (type I complex), or CD124 combined with CD213a1 (type II complex).The 11B11 antibody is widely used for detection of intracellular levels of IL-4 protein by flow cytometry, as well as for analysis of soluble cytokine as measured by ELISA, and in functional assays to neutralization cytokine-receptor interactions. Please choose the appropriate format for each application.

Mouse Anti-IL-4 / Interleukin-4 Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-IL-4 / Interleukin-4 Antibody Clone: 11B11
Clone 11B11 Host and Isotype: Rat IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 0.2 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

IL-4 / Interleukin-4 Previously Observed Antibody Staining Patterns

Selected References

Cook PC, Jones LH, Jenkins SJ, Wynn TA, Allen JE, and MacDonald AS. 2012. Proc. Natl. Acad. Sci. 109: 9977-9982. (in vivo blocking)Altin JA, Goodnow CC, and Cook MC. 2012. J. Immunol. 5478-5488. (Flow cytometry)Tofukuji S, Kuwahara M, Suzuki J, Ohara O, Nakayama T, and Yamashita M. 2012. J. Immunol. 188: 4846-4857. (in vitro Th1 polarization)Weber KS, Hildner K, Murphy KM and Allen PM. 2010 J. Immunol. 185: 2836-2846 (in vitro Th1 polarization, ELISA)Odobasic D, Kitching AR, Semple TJ, Timoshanko JR, Tipping PG, and Holdsworth SR. 2005. J. Am. Soc. Nephrol. 16: 2012-2022. (in vivo activation, Immunofluorescence microscopy - frozen tissue, Immunohistochemistry - frozen tissue)

Limitations and Warranty

enQuire Bio's Mouse Anti-IL-4 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-IL-4 Antibody [11B11]