Human PBMCs were stimulated with PMA and Ionomycin and stained with APC Human Anti-CD3 , followed by intracellular staining with 5 uL  (right panel) or 0.125 ug PE Rat IgG1 (left panel).

Anti-IL-4 Antibody [MP4-25D2]

$ 78.00$ 198.00
Please Select Product Options Below To View The Catalog Number.

Species: Human
Tested Applications: Flow Cytometry
Available Conjugates: APC ;PE
Isotype: Rat IgG1 kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: QAB82-APC-25Tests
IL-4 / Interleukin-4 General Information
Alternate Names
IL4, Interleukin-4, BSF-1, BSF1, IL-4, BCGF1, BCGF-1
Curated Database and Bioinformatic Data
Gene SymbolIL4
Entrez Gene ID3565
Ensemble Gene IDENSG00000113520
RefSeq Protein Accession(s)NP_000580, NP_001341919, NP_758858
RefSeq mRNA Accession(s)NM_172348, NM_000589, NM_001354990,
RefSeq Genomic Accession(s)NG_023252, NC_018916, NC_000005,
UniProt ID(s)Q5FC01, D4HNR6, P05112
UniGene ID(s)Q5FC01, D4HNR6, P05112
HGNC ID(s)6014
Cosmic ID(s)IL4
KEGG Gene ID(s)hsa:3565
PharmGKB ID(s)PA197
General Description of IL-4 / Interleukin-4.
The MP4-25D2 antibody is specific for human IL-4, a pleiotropic cytokine that Th2 cell differentiation, B cell proliferation and differentiation, and B cell class switching to IgE. MP4-25D2 is a neutralizing antibody and is reported to cross react with rhesus monkey IL-4.

Human Anti-IL-4 / Interleukin-4 Antibody Product Attributes

Species: Human
Cross Reactivity: rhesus macaque
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1x105 to 1x108 cells.
Clonality: Monoclonal Antibody
Anti-IL-4 / Interleukin-4 Antibody Clone: MP4-25D2
Clone MP4-25D2 Host and Isotype: Rat IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

IL-4 / Interleukin-4 Previously Observed Antibody Staining Patterns

Selected References

Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, and Silver JE. 1992. Immunol Rev. 127:5-24. Chretien I, Van Kimmenade A, Pearce MK, Banchereau J, and Abrams JS. 1989. J Immunol Methods. 117(1):67-71. Jung T, Schauer U, Rieger C, et al. 1995. Eur J Immunol. 25(8):2413-2416. Prussin C and Metcalfe DD. 1995. J Immunol Methods. 188(1):117-128.

Limitations and Warranty

enQuire Bio's Human Anti-IL-4 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-IL-4 Antibody [MP4-25D2]