Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with IL-6 Monoclonal Antibody (IL6/127).
Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with IL-6 Monoclonal Antibody (IL6/127).

Anti-IL-6 / Interferon beta-2 Antibody Clone IL6/1270

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: 3569-RTM1
Species: Human and Mouse
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Rat IgG1 kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 3569-RTM1-P1
IL-6 / Interferon beta-2 General Information
Alternate Names
Interleukin 6, IL-6, IL6
Molecular Weight
21kDa
Chromosomal Location
7p21
Curated Database and Bioinformatic Data
Gene SymbolIL6
Entrez Gene ID3569
Ensemble Gene IDENSG00000136244
RefSeq Protein Accession(s)XP_005249802, XP_011513692, NP_001305024, NP_000591
RefSeq mRNA Accession(s)XM_005249745, NM_000600, XM_011515390 NM_001318095
RefSeq Genomic Accession(s)NC_000007, NG_011640, NC_018918
UniProt ID(s)B4DVM1, P05231, Q75MH2, B4DNQ5, B5MC21
UniGene ID(s)B4DVM1, P05231, Q75MH2, B4DNQ5, B5MC21
HGNC ID(s)6018
Cosmic ID(s)IL6
KEGG Gene ID(s)hsa:3569
PharmGKB ID(s)PA198
General Description of IL-6 / Interferon beta-2.
IL-6 is a potent lymphoid cell growth factor that stimulates the growth, survivability of certain B-cells, T-cells. It plays a critical role in B-cell differentiation to plasma cells, is a potent growth factor for plasmacytoma, myeloma. IL-6 is produced by a variety of cell types, including monocytes, fibroblasts, endothelial cells. Upon stimulation, macrophages, T, B, mast,, glial cells, eosinophils, keratinocytes, granulocytes also secrete IL-6. It is involved in host defense, acute phase reactions, immune responses,, hematopoiesis.

Human and Mouse Anti-IL-6 / Interferon beta-2 Antibody Product Attributes

Species: Human and Mouse
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (1-2ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-IL-6 / Interferon beta-2 Antibody Clone: IL6/1270
Clone IL6/1270 Host and Isotype: Rat IgG1 kappa
Anti-Human and Mouse IL-6 / Interferon beta-2 Positive Control Sample: Heart
Cellular Localization of Antibody Cytoplasmic, extracellular (secreted)
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant IL-6 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

IL-6 / Interferon beta-2 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-IL6 antibody staining is expected to be primarily localized to the vesicles.

Observed Antibody Staining Data By Tissue Type:

Variations in IL-6 / Interferon beta-2 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of IL-6 / Interferon beta-2 could be seen inlymphoid tissue in appendix. We were unable to detect IL-6 / Interferon beta-2 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Limitations and Warranty

enQuire Bio's IL-6 / Interferon beta-2 Anti-Human, Mouse Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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