C57Bl/6 splenocytes were stimulated with PMA and Ionomycin , followed by intracellular staining with 0.06 ug APC Mouse Anti-IL-2 (QAB80).

Mouse Anti-IL-2 Antibody [JES6-5H4]

$ 59.00$ 219.00
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SKU: QAB80
Species: Mouse
Tested Applications: Flow Cytometry
Available Conjugates: Biotin ;APC ;PE
Isotype: Rat IgG2b kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: QAB80-B-25ug
Interleukin-2 / IL-2 General Information
Alternate Names
Interleukin-2, IL-2 Protein, Il-2
Curated Database and Bioinformatic Data
Gene SymbolIl2
Entrez Gene ID16183
Ensemble Gene IDENSMUSG00000027720
RefSeq Protein Accession(s)NP_032392
RefSeq mRNA Accession(s)NM_008366
RefSeq Genomic Accession(s)NG_039252, NC_000069, NG_039248
UniProt ID(s)P04351
UniGene ID(s)P04351
Cosmic ID(s)Il2
KEGG Gene ID(s)mmu:16183
General Description of Interleukin-2 / IL-2.
The JES6-5H4 antibody binds to mouse Interleukin-2 (IL-2), a 17 kDa cytokine that is secreted by activated T cells. This cytokine acts as an autocrine stimulatory factor for T cell proliferation, differentiation and survival, as well as being an effective activator of B cells and inducer of NK cell cytotoxic functions. IL-2 plays a key modulatory role in the differentiation of T cells toward either Th17 or T regulatory (Treg) cell types and is important for Treg cell function. The IL-2 receptor (IL-2R) is a complex consisting of an IL-2R alpha chain (CD25), along with the IL-2R beta chain (CD122) and the common gamma chain (CD132). IL-2 may also bind with low affinity to the T cell surface protein CD25 alone, although this interaction does not induce cell signaling.The JES6-5H4 antibody is extensively used for intracellular detection of IL-2 by flow cytometry, for detection of soluble cytokine in ELISA, and as a neutralizing reagent in vitro and in vivo.

Mouse Anti-Interleukin-2 / IL-2 Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-Interleukin-2 / IL-2 Antibody Clone: JES6-5H4
Clone JES6-5H4 Host and Isotype: Rat IgG2b kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH 7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

Interleukin-2 / IL-2 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Interleukin-2 / IL-2 expression as measured by anti-Interleukin-2 / IL-2 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity + - + ++ + - + - - - - + + ++ - - + - + +
IL2 Variability ++ ++ ++ ++ ++ ++ + ++ ++ ++ ++ ++ ++ + + + ++ ++ +++ ++

Selected References

Attridge K, Wang CJ, Wardzinski L, Kenefeck R, Chamberlain JL, Manzotti C, Kopf M, and Walker LSK. 2012. Blood. 119: 4656-4664. (Flow cytometry)Helbig C, Gentek R, Backer RA, de Souza Y, Derks IAM, Eldering E, Wagner K, Jankovic D, Gridley T, Moerland PD, Flavell RA, and Amsen D. 2012. Proc. Natl. Acad. Sci. 109: 9041-9046. (ELISA - detection)Chen Q, Kiim YC, Laurence A, Punkosdy GA, and Shevach EM. 2011. J. Immunol. 186: 6329-6337. (in vivo blocking)Weber KS, Hildner K, Murphy KM and Allen PM. 2010 J. Immunol. 185: 2836-2846. (ELISA)Wang C, Morley SC, Donermeyer D, Peng I, Lee WP, Devoss J, Damilenko DM, Lin Z, Zhang J, Zhou J, Allen PM, and Brown EJ. 2010. J. Immunol. 185: 7487-7497. (Immunocytochemistry/Immunofluorescence microscopy)Cardona AE and Teale JM. 2002. J. Immunol. 169: 3163-3171. (Immunohistochemistry - frozen tissue)Woo AL, Gildea LA, Tack LM, Miller ML, Spicer A, Millhorn DE, Finkelman FD, Dassett DJ, and Shull GE. 2002. J. Biol. Chem. 277: 49036-49046. (in vivo blocking)

Limitations and Warranty

enQuire Bio's Mouse Anti-IL-2 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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