Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Laminin Monoclonal Antibody (A5).
Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Laminin Monoclonal Antibody (A5).

Anti-Laminin, gamma 1 Antibody Clone A5

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: 3915-RTM1
Species: Human and Mouse
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Rat IgG2a kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 3915-RTM1-P1
Laminin, gamma 1 General Information
Alternate Names
Laminin subunit gamma-1, LAMC1
Molecular Weight
210kDa
Chromosomal Location
1q31
Curated Database and Bioinformatic Data
Gene SymbolLAMC1
Entrez Gene ID3915
Ensemble Gene IDENSG00000135862
RefSeq Protein Accession(s)NP_002284
RefSeq mRNA Accession(s)NM_002293
RefSeq Genomic Accession(s)NG_011463, NC_018912, NC_000001
UniProt ID(s)Q6NVY8, P11047
UniGene ID(s)Q6NVY8, P11047
HGNC ID(s)6492
Cosmic ID(s)LAMC1
KEGG Gene ID(s)hsa:3915
PharmGKB ID(s)PA30280
General Description of Laminin, gamma 1.
Laminins are large hetero-trimeric, non-collagenous glycoproteins composed of alpha, beta,, γ chains. This MAb reacts with laminin B2/1 chain of ~210kDa, does not cross-react with other basement membrane components or fibronectin. Its specificity was established by immunoprecipitation, immunofluorescence of human skeletal muscle, kidney with laminin chain-specific MAbs. Epithelial sheets in vivo are separated from the mesenchymal elements of the stroma by a thin layer of a specialized type of extracellular matrix termed the basement membrane (BM). This structure consists of individual components, some of which are ubiquitous in BMs, some are not. The ubiquitous ones comprise laminin (LN), entactin/niCanineen (EN), collagen type IV (CIV),, large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous, regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human, animal epithelial neoplasms. This MAb stains uniformly all human, murine basement membranes.

Human and Mouse Anti-Laminin, gamma 1 Antibody Product Attributes

Species: Human and Mouse
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 min at RT)
Clonality: Monoclonal
Anti-Laminin, gamma 1 Antibody Clone: A5
Clone A5 Host and Isotype: Rat IgG2a kappa
Anti-Human and Mouse Laminin, gamma 1 Positive Control Sample: Normal colon or carcinomas
Cellular Localization of Antibody Basement membrane
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Murine EHS laminin preparation
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Laminin, gamma 1 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-LAMC1 antibody staining is expected to be primarily localized to the endoplasmic reticulum and plasma membrane.

Observed Antibody Staining Data By Tissue Type:

Variations in Laminin, gamma 1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in trophoblastic cells in the placenta. More moderate antibody staining intensity was present in trophoblastic cells in the placenta. Low, but measureable presence of Laminin, gamma 1 could be seen inadipocytes in mesenchymal tissue, cells in the endometrial stroma in endometrium, cells in the seminiferous ducts in testis, cells in the white pulp in spleen, epidermal cells in the skin, fibroblasts in mesenchymal tissue, glandular cells in the appendix, colon, endometrium, gallbladder, prostate, rectum, salivary gland, stomach and thyroid gland, keratinocytes in skin, Leydig cells in the testis, neuronal cells in the caudate nucleus, ovarian stroma cells in the ovary, peripheral nerve in mesenchymal tissue, respiratory epithelial cells in the nasopharynx, squamous epithelial cells in the cervix and uterine, oral mucosa and vagina. We were unable to detect Laminin, gamma 1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Laminin, gamma 1 expression as measured by anti-Laminin, gamma 1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - - - - - - - - - - - - - ++ - -
LAMC1 Variability + + + + + + ++ + ++ + ++ + + + + + + ++ + +

Limitations and Warranty

enQuire Bio's Laminin, gamma 1 Anti-Human, Mouse Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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