Human and Mouse Anti-Laminin, gamma 1 Antibody Product Attributes
Species: Human and Mouse
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 min at RT)
Clonality: Monoclonal
Anti-Laminin, gamma 1 Antibody Clone: A5
Clone A5 Host and Isotype: Rat IgG2a kappa
Anti-Human and Mouse Laminin, gamma 1 Positive Control Sample: Normal colon or carcinomas
Cellular Localization of Antibody Basement membrane
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Murine EHS laminin preparation
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Laminin, gamma 1 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-LAMC1 antibody staining is expected to be primarily localized to the endoplasmic reticulum and plasma membrane.Observed Antibody Staining Data By Tissue Type:
Variations in Laminin, gamma 1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in trophoblastic cells in the placenta. More moderate antibody staining intensity was present in trophoblastic cells in the placenta. Low, but measureable presence of Laminin, gamma 1 could be seen inadipocytes in mesenchymal tissue, cells in the endometrial stroma in endometrium, cells in the seminiferous ducts in testis, cells in the white pulp in spleen, epidermal cells in the skin, fibroblasts in mesenchymal tissue, glandular cells in the appendix, colon, endometrium, gallbladder, prostate, rectum, salivary gland, stomach and thyroid gland, keratinocytes in skin, Leydig cells in the testis, neuronal cells in the caudate nucleus, ovarian stroma cells in the ovary, peripheral nerve in mesenchymal tissue, respiratory epithelial cells in the nasopharynx, squamous epithelial cells in the cervix and uterine, oral mucosa and vagina. We were unable to detect Laminin, gamma 1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of Laminin, gamma 1 expression as measured by anti-Laminin, gamma 1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.| Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal Intensity | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | ++ | - | - |
| LAMC1 Variability | + | + | + | + | + | + | ++ | + | ++ | + | ++ | + | + | + | + | + | + | ++ | + | + |
Limitations and Warranty
enQuire Bio's Laminin, gamma 1 Anti-Human, Mouse Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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