Human, Rat, and Canine Anti-MAGE-1 Antibody Product Attributes
Species: Human, Rat, and Canine
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Anti-MAGE-1 Antibody Clone: SPM282
Clone SPM282 Host and Isotype: Mouse IgG1 kappa
Anti-Human, Rat, and Canine MAGE-1 Positive Control Sample: Melanoma cell lines. Melanomas, gliomas, neuroblastoma, non-small cell lung cancer, breast, gastric, colorectal, ovarian,, renal cell carcinomas.
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Human MAGE-A1 full length recombinant protein
Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.
MAGE-1 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-MAGEA1 antibody staining is expected to be primarily localized to the cytosol.
Observed Antibody Staining Data By Tissue Type:
Variations in MAGE-1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the seminiferous ducts in testis. More moderate antibody staining intensity was present in cells in the seminiferous ducts in testis. Low, but measureable presence of MAGE-1 could be seen in. We were unable to detect MAGE-1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Limitations and Warranty
enQuire Bio's MAGE-1 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.