Formalin-fixed, paraffin-embedded Human Colon Carcinoma stained with MAML2 Monoclonal Antibody (MAML2/132).
Formalin-fixed, paraffin-embedded Human Colon Carcinoma stained with MAML2 Monoclonal Antibody (MAML2/132).Formalin-fixed, paraffin-embedded Human Bladder Carcinoma stained with MAML2 Monoclonal Antibody (MAML2/132).Formalin-fixed, paraffin-embedded Human Placenta stained with MAML2 Monoclonal Antibody (MAML2/132).

Anti-MAML2 Antibody Clone MAML2/1302

$ 228.00$ 518.00
Please Select Product Options Below To View The Catalog Number.

SKU: 84441-MSM1
Species: Human
Tested Applications: Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2a
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
84441-MSM1-P0 Size: 20 ug, Tag: Unconjugated $ 228.00
84441-MSM1-P1 Size: 100 ug, Tag: Unconjugated $ 488.00
84441-MSM1-P2 Size: 200 ug, Tag: Unconjugated $ 518.00
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In Stock.
Flat Rate Shipping Anywhere in the US: $45
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SKU: 84441-MSM1-P0
MAML2 General Information
Alternate Names
Mastermind-like protein 2, MAML2
Molecular Weight
125kDa
Chromosomal Location
11q21
Curated Database and Bioinformatic Data
Gene SymbolMAML2
Entrez Gene ID84441
Ensemble Gene IDENSG00000184384
RefSeq Protein Accession(s)XP_011541327, NP_115803, XP_011541326, XP_011541325
RefSeq mRNA Accession(s)XM_011543024, XM_011543025, XM_011543023, NM_032427
RefSeq Genomic Accession(s)NC_000011, NC_018922
UniProt ID(s)Q8IZL2, Q6Y3A3, Q8IUL3
UniGene ID(s)Q8IZL2, Q6Y3A3, Q8IUL3
HGNC ID(s)16259
Cosmic ID(s)MAML2
KEGG Gene ID(s)hsa:84441
PharmGKB ID(s)PA134946327, PA30730
General Description of MAML2.
MAML2 (mastermind-like protein 2), also known as MAM2, MAM3 or MLLMAML2, is a nuclear speckle protein that acts as a transcriptional co-activator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML2 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, MAML2 interacts with the ankyrin repeats of the Notch proteins Notch 1, Notch 2, Notch 3 and Notch 4. This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-J? ;a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML2 is essential for proper Notch binding, the C-terminal domain of MAML2 is essential for transcriptional activation. A chromosomal aberration involving the gene encoding MAML2 is implicated in mucoepidermoid carcinomas, clear cell hidradenomas and benign Warthin tumors.

Human Anti-MAML2 Antibody Product Attributes

Species: Human
Tested Applications: Immunohistochemistry (IHC).
Clonality: Monoclonal
Anti-MAML2 Antibody Clone: MAML2/1302
Clone MAML2/1302 Host and Isotype: Mouse IgG2a
Anti-Human MAML2 Positive Control Sample: MCF-7 cells. Pancreas or Placenta. Bladder or Colon Carcinoma.
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA and 0.05% Azide
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human full-length MAML2
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

MAML2 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-MAML2 antibody staining is expected to be primarily localized to the nuclear bodies, nucleoplasm and cytosol. There is variability in either the signal strength or the localization of signal in nuclear bodies from cell to cell.

Observed Antibody Staining Data By Tissue Type:

Variations in MAML2 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the endometrial stroma in endometrium, cells in the glomeruli in kidney, cells in the tubules in kidney, glandular cells in the adrenal gland, colon, duodenum, prostate, salivary gland and stomach, Leydig cells in the testis, macrophages in lung, Purkinje cells in the cerebellum, respiratory epithelial cells in the bronchus, squamous epithelial cells in the esophagus, oral mucosa and tonsil and urothelial cells in the urinary bladder. More moderate antibody staining intensity was present in cells in the endometrial stroma in endometrium, cells in the glomeruli in kidney, cells in the tubules in kidney, glandular cells in the adrenal gland, colon, duodenum, prostate, salivary gland and stomach, Leydig cells in the testis, macrophages in lung, Purkinje cells in the cerebellum, respiratory epithelial cells in the bronchus, squamous epithelial cells in the esophagus, oral mucosa and tonsil and urothelial cells in the urinary bladder. Low, but measureable presence of MAML2 could be seen inadipocytes in breast, cells in the granular layer in cerebellum, cells in the molecular layer in cerebellum, cells in the red pulp in spleen, cells in the white pulp in spleen, germinal center cells in the tonsil, glandular cells in the endometrium and epididymis, glial cells in the caudate nucleus, hematopoietic cells in the bone marrow, neuronal cells in the hippocampus and neuropil in cerebral cortex. We were unable to detect MAML2 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of MAML2 expression as measured by anti-MAML2 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity + + ++ ++ ++ - + ++ - + + ++ ++ ++ + ++ + + ++ +
MAML2 Variability ++ ++ ++ ++ ++ ++ ++ +++ ++ ++ ++ + +++ ++ +++ ++ ++ ++ ++ ++

Limitations and Warranty

enQuire Bio's MAML2 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-MAML2 Antibody Clone MAML2/1302

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