Formalin-fixed, paraffin-embedded Human Colon Carcinoma stained with MAML3 Monoclonal Antibody (MAML3/133).
Formalin-fixed, paraffin-embedded Human Colon Carcinoma stained with MAML3 Monoclonal Antibody (MAML3/133).Formalin-fixed, paraffin-embedded Human Cervical Carcinoma stained with MAML3 Monoclonal Antibody (MAML3/133).Formalin-fixed, paraffin-embedded Human Placenta stained with MAML3 Monoclonal Antibody (MAML3/133).

Anti-MAML3 Antibody Clone MAML3/1303

$ 199.00$ 459.00
Please Select Product Options Below To View The Catalog Number.

SKU: 55534-MSM1
Species: Human
Tested Applications: Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
55534-MSM1-P0 Size: 20 ug, Tag: Unconjugated $ 199.00
55534-MSM1-P1 Size: 100 ug, Tag: Unconjugated $ 429.00
55534-MSM1-P2 Size: 200 ug, Tag: Unconjugated $ 459.00
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In Stock.
Flat Rate Shipping Anywhere in the US: $45
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SKU: 55534-MSM1-P0
MAML3 General Information
Alternate Names
MAML3, Mastermind Like Transcriptional Coactivator 3
Molecular Weight
150-170kDa
Chromosomal Location
4q28
Curated Database and Bioinformatic Data
Gene SymbolMAML3
Entrez Gene ID55534
Ensemble Gene IDENSG00000196782
RefSeq Protein Accession(s)NP_061187
RefSeq mRNA Accession(s)NM_018717
RefSeq Genomic Accession(s)NC_000004, NC_018915
UniProt ID(s)Q9NPV6, Q96JK9
UniGene ID(s)Q9NPV6, Q96JK9
HGNC ID(s)16272
Cosmic ID(s)MAML3
KEGG Gene ID(s)hsa:55534
PharmGKB ID(s)PA134953776
General Description of MAML3.
MAML3 (mastermind-like protein 3) is a nuclear speckle protein that acts as a transcriptional coactivator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML3 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, MAML3 interacts with the ankyrin repeats of the Notch proteins Notch 1, Notch 2, Notch 3 and Notch 4.This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-J? ;a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML3 is essential for proper Notch binding, the C-terminal domain of MAML3 is essential for transcriptional activation. Due to its involvement in cell signaling and transcriptional activation, upregulation of MAML3 is thought to be involved in oncogenesis.

Human Anti-MAML3 Antibody Product Attributes

Species: Human
Tested Applications: Immunohistochemistry (IHC).
Clonality: Monoclonal
Anti-MAML3 Antibody Clone: MAML3/1303
Clone MAML3/1303 Host and Isotype: Mouse IgG1
Anti-Human MAML3 Positive Control Sample: MCF-7 cells. Pancreas or Placenta. Cervix or Colon Carcinoma.
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA and 0.05% Azide
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human full-length MAML3 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

MAML3 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-MAML3 antibody staining is expected to be primarily localized to the nuclear interchromatin granular clusters.

Observed Antibody Staining Data By Tissue Type:

Variations in MAML3 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in adipocytes in breast and mesenchymal tissue, chondrocytes in mesenchymal tissue, endothelial cells in the colon, epidermal cells in the skin, fibroblasts in skin and mesenchymal tissue, glandular cells in the colon, duodenum, gallbladder, rectum, salivary gland, stomach and thyroid gland, hematopoietic cells in the bone marrow, keratinocytes in skin, melanocytes in skin, peripheral nerve/ganglion in colon, squamous epithelial cells in the esophagus and oral mucosa and trophoblastic cells in the placenta. More moderate antibody staining intensity was present in adipocytes in breast and mesenchymal tissue, chondrocytes in mesenchymal tissue, endothelial cells in the colon, epidermal cells in the skin, fibroblasts in skin and mesenchymal tissue, glandular cells in the colon, duodenum, gallbladder, rectum, salivary gland, stomach and thyroid gland, hematopoietic cells in the bone marrow, keratinocytes in skin, melanocytes in skin, peripheral nerve/ganglion in colon, squamous epithelial cells in the esophagus and oral mucosa and trophoblastic cells in the placenta. Low, but measureable presence of MAML3 could be seen inbile duct cells in the liver, cells in the red pulp in spleen, germinal center cells in the tonsil, glandular cells in the parathyroid gland, glial cells in the caudate nucleus and hippocampus, neuronal cells in the caudate nucleus and cerebral cortex, peripheral nerve in mesenchymal tissue and Purkinje cells in the cerebellum. We were unable to detect MAML3 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Limitations and Warranty

enQuire Bio's MAML3 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-MAML3 Antibody Clone MAML3/1303

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