Antibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:50-1:100 |
| Pretreatment | Citrate Buffer pH 6.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Clonality: Monoclonal
Anti-MART-1 Antibody Clone: EP43
Host and Isotype: Rabbit Rabbit IgG
Recommended Positive Control Sample: Skin, Melanoma
Cellular Localization of Antibody EP43 Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: A synthetic peptide corresponding to residues on the N-terminus of human MART-1 protein
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
MART-1 Information for Pathologists
Summary:
Melanocyte specific cytoplasmic protein involved in the formation of stage II melanosomes. MelanA was discovered as an antigen recognized by tumor infiltrating cytotoxic T cells from a melanoma patient, hence the name Melanoma Antigen (J Exp Med 1994;180:35). Same gene was independently identified by a different research group who named it MART1 (Melanoma Antigen Recognized by T cells 1) (Proc Natl Acad Sci USA 1994;91:3515); MelanA and MART1 are synonyms. Two antibodies are commercially available: A103 and M2-7C10. A103: mouse monoclonal antibody against MelanA recombinant protein (Proc Natl Acad Sci USA 1996;93:5915, US patent 5,674,749).Common Uses By Pathologists:
To establish the diagnosis of metastatic melanoma, more sensitive than anti-gp100 antibody (HMB45) (Am J Surg Pathol 1998;22:976). Used in conjunction with S100 as a standard panel for evaluating sentinel lymph nodes for melanoma; HMB45 can be added to the panel but is not essential (Am J Surg Pathol 2001;25:1039). To confirm the diagnosis of angiomyolipoma, lymphangioleiomyomatosis (LAM) and other PEComas. To distinguish adrenal cortical tumors from renal cell carcinoma (clone A103 only); inhibin A can also be used for this purpose. To confirm the diagnosis of a sex cord stromal tumor (clone A103 only); inhibin A can also be used for this purpose.Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
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