Human, Mouse, and Rat Anti-MART-1 / Melan-A / MLANA Antibody Product Attributes
Species: Human, Mouse, and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Anti-MART-1 / Melan-A / MLANA Antibody Clone: SPM540
Clone SPM540 Host and Isotype: Mouse IgG2b kappa
Anti-Human, Mouse, and Rat MART-1 / Melan-A / MLANA Positive Control Sample: SK-MEL-13, SK-MEL-19 Melanoma cell lines; Melanomas
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant hMART-1 protein
Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.
MART-1 / Melan-A / MLANA Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:
Variations in MART-1 / Melan-A / MLANA antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in melanocytes in skin. More moderate antibody staining intensity was present in melanocytes in skin. Low, but measureable presence of MART-1 / Melan-A / MLANA could be seen in. We were unable to detect MART-1 / Melan-A / MLANA in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Limitations and Warranty
enQuire Bio's MART-1 / Melan-A / MLANA Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.