Staining by anti-MART-1 / Melan-A / MLANA Antibody 1

Anti-MART-1 / Melan-A / MLANA Antibody Clone SPM540

$ 429.00
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SKU: 2315-MSM2X
Species: Human, Mouse, and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2b kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
2315-MSM2X-P1 Size: 100 ug, Tag: Unconjugated
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SKU: 2315-MSM2X-P1
MART-1 / Melan-A / MLANA General Information
Alternate Names
Protein melan-A melanoma antigen recognized by T cells 1, MART-1, MLANA
Molecular Weight
20-22kDa (doublet)
Chromosomal Location
Curated Database and Bioinformatic Data
Gene SymbolMLANA
Entrez Gene ID2315
Ensemble Gene IDENSG00000120215
RefSeq Protein Accession(s)NP_005502
RefSeq mRNA Accession(s)NM_005511
RefSeq Genomic Accession(s)NC_018920, NC_000009
UniProt ID(s)Q16655
UniGene ID(s)Q16655
HGNC ID(s)7124
Cosmic ID(s)MLANA
KEGG Gene ID(s)hsa:2315
PharmGKB ID(s)PA30842
General Description of MART-1 / Melan-A / MLANA.
This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1,, GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes, endoplasmic reticulum. This MAb labels melanomas, other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Human, Mouse, and Rat Anti-MART-1 / Melan-A / MLANA Antibody Product Attributes

Species: Human, Mouse, and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-MART-1 / Melan-A / MLANA Antibody Clone: SPM540
Clone SPM540 Host and Isotype: Mouse IgG2b kappa
Anti-Human, Mouse, and Rat MART-1 / Melan-A / MLANA Positive Control Sample: SK-MEL-13, SK-MEL-19 Melanoma cell lines; Melanomas
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant hMART-1 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

MART-1 / Melan-A / MLANA Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in MART-1 / Melan-A / MLANA antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in melanocytes in skin. More moderate antibody staining intensity was present in melanocytes in skin. Low, but measureable presence of MART-1 / Melan-A / MLANA could be seen in. We were unable to detect MART-1 / Melan-A / MLANA in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Limitations and Warranty

enQuire Bio's MART-1 / Melan-A / MLANA Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-MART-1 / Melan-A / MLANA Antibody Clone SPM540