Immunohistochemical staining of Melanoma Cocktail Antibody  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

Melanoma Cocktail Antibody [HMB45+A103+T311] Antibody (cGMP).

$ 135.00$ 1,055.00
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Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Mouse HMB45 and A103, IgG1; T311, IgG2a

Product NumberDescriptionPrice
QIVD295-0.1ml Size: 0.1 ml, Format: Concentrate $ 135.00
QIVD295-0.5ml Size: 0.5 ml, Format: Concentrate $ 635.00
QIVD295-1ml Size: 1 ml, Format: Concentrate $ 1,055.00
QIVD295-6ml Size: 6 ml, Format: Predilute $ 375.00
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Datasheets and Documentation
Product Datasheet
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SKU: QIVD295-0.1ml
Melanoma Cocktail Antibody General Information
Alternate Names
Curated Database and Bioinformatic Data
UniProt ID(s)Uncharacterized Antigen
General Description of Melanoma Cocktail Antibody .
The HMB45 clone reacts with a neuraminidase-sensitive oligosaccharide side chain of a glycoconjugate present in immature melanosomes. The HMB45-reactive antigen is present in cutaneous melanocytes, prenatal and infantile retinal pigment epithelium and melanoma cells and is thought to be oncofetal in nature. This antibody has been shown to label the majority of melanomas. Clone A103 recognizes a protein of 20 kDa, identified as MART-1 (melanoma antigen recognized by T cells-1) or Melan-A. Melan-A is a useful addition to melanoma panels as it is apparently specific for melanocytic lesions. Studies have also shown that MART-1 is more sensitive than HMB45 when labeling metastatic melanomas. Tyrosinase is a key enzyme involved in the initial stages of melanin biosynthesis. Studies have shown tyrosinase to be a more sensitive marker when compared to HMB45 and MART-1. It has been shown to label a higher percentage of desmoplastic melanomas than HMB45. The combination of HMB45, MART-1 and tyrosinase make this triple antibody cocktail a first-order pan melanoma screener.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:25-1:50
Pretreatment Citrate Buffer pH 6.0
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-Melanoma Cocktail Antibody Antibody Clone: HMB45+A103+T311
Host and Isotype: Mouse HMB45 and A103, IgG1; T311, IgG2a
Recommended Positive Control Sample: Metastatic melanoma in lymph node
Cellular Localization of Antibody HMB45+A103+T311 Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: Pigmented melanoma metastases from lymph nodes (HMB45). Recombinant human MART-1 protein (A103). Recombinant tyrosinase protein T311.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

Melanoma Cocktail Antibody Information for Pathologists

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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Melanoma Cocktail Antibody [HMB45+A103+T311] Antibody (cGMP).