C57Bl/6 splenocytes were stained with 0.06 ug Mouse Anti-MHC Class II APC (QAB70) (solid line) or 0.06 ug Rat IgG2b APC isotype control (dashed line). Flow Cytometry Data from 10,000 events.
C57Bl/6 splenocytes were stained with 0.06 ug Mouse Anti-MHC Class II APC (QAB70) (solid line) or 0.06 ug Rat IgG2b APC isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 splenocytes were stained with 0.5 ug APC-Cy7 Mouse Anti-MHC Class II .C57Bl/6 splenocytes were stained with 0.25 ug PerCP-Cy5.5 Mouse Anti-MHC Class II .C57Bl/6 splenocytes were stained with 0.06 ug Mouse Anti-MHC Class II PE (QAB70) (solid line) or 0.06 ug Rat IgG2b PE isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 splenocytes were stained with 0.5 ug Qfluor™ 710  Mouse Anti-MHC Class II (QAB70) (solid line) or 0.5 ug Qfluor™ 710  Rat IgG2b isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 splenocytes were stained with 0.5 ug V450  Mouse Anti-MHC Class II  (solid line) or 0.5 ug V450 Rat IgG2b isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 splenocytes were stained with 0.06 ug Mouse Anti-MHC Class II APC (QAB70) (solid line) or 0.06 ug Rat IgG2b APC isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 splenocytes were stained with 0.5 ug APC-Cy7 Mouse Anti-MHC Class II .C57Bl/6 splenocytes were stained with 0.25 ug PerCP-Cy5.5 Mouse Anti-MHC Class II .C57Bl/6 splenocytes were stained with 0.06 ug Mouse Anti-MHC Class II PE (QAB70) (solid line) or 0.06 ug Rat IgG2b PE isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 splenocytes were stained with 0.5 ug Qfluor™ 710  Mouse Anti-MHC Class II (QAB70) (solid line) or 0.5 ug Qfluor™ 710  Rat IgG2b isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 splenocytes were stained with 0.5 ug V450  Mouse Anti-MHC Class II  (solid line) or 0.5 ug V450 Rat IgG2b isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Anti-MHC Class II Antibody (I-A/I-E) [M5/114.15.2]

$ 48.00$ 348.00
Please Select Product Options Below To View The Catalog Number.

SKU: QAB70
Species: Mouse
Tested Applications: Flow Cytometry
Available Conjugates: FITC ;Unconjugated ;APC ;APC-Cy7 ;PerCP-Cy5.5 ;PE ;Qfluor 710 ;V450
Isotype: Rat IgG2b kappa
Mass Spec Validated?: Not MS Validated

Size
Tag Clear selection
Shipping Information
In Stock: 1-2 Day Lead Time.
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
SKU: QAB70-F-25ug
MHC class II antigen General Information
Alternate Names
histocompatibility 2, class II antigen A, beta 1, histocompatibility 2, class II antigen E, beta, H2Ab1, H2Eb1, H2-Ab1, H2-Eb1, MHC II
Curated Database and Bioinformatic Data
Gene SymbolH2-Ab1 and H2-Eb1
Entrez Gene ID14961, 14969
Ensemble Gene IDENSMUSG00000073421, ENSMUSG00000060586
RefSeq Protein Accession(s)NP_996988, NP_034512
RefSeq mRNA Accession(s)NM_207105, NM_010382
RefSeq Genomic Accession(s)NG_187027, NC_000083, NG_187004, NG_039662NG_187004, NC_000083, NG_187027, NG_039662
UniProt ID(s)Q3TD53, P14483, Q8BPA0, O78196
UniGene ID(s)Q3TD53, P14483, Q8BPA0, O78196
Cosmic ID(s)H2-Ab1, H2-Eb1
KEGG Gene ID(s)mmu:14961, mmu:14969
General Description of MHC class II antigen.
The M5/114.15.2 antibody reacts with mouse MHC Class II alloantigens I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek, as well as being cross-reactive with mouse cells of H-2p and H-2r haplotype. MHC Class II is widely expressed by mouse immune cells bearing these alloantigens, including T and B cells, monocytes, macrophages, and dendritic cells. The antibody does not react with the following alloantigens: I-Af, I-Ak, I-As, or NOD H-2g7.The M5/114.15.2 antibody may be used for analysis of mouse cells expressing MHC Class II alloantigens as described. Please note that the M5/114.15.2 clone may also be referred to as M5/114 in the literature.

Mouse Anti-MHC class II antigen Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-MHC class II antigen Antibody Clone: M5/114.15.2
Clone M5/114.15.2 Host and Isotype: Rat IgG2b kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

MHC class II antigen Previously Observed Antibody Staining Patterns

Selected References

Staehli F, Ludigs K, Heinz LX, Segin-Estevez Q, Ferrero I, Braun M, Schroder K, Rebsamen M, Tardivel A, Mattmann C, MacDonald HR, Romero P, Reith W, Guarda G, and Tschopp J. 2012. J. Immunol. 188: 3820-3828. (Flow cytometry)Parra D, Rieger AM, Li J, Zhang Y-A, Randall LM, Hunter CA, Barreda DR, and Sunyer JO. 2012. J. Leukoc. Biol. 91:525-536. (in vitro blocking, Flow cytometry)Scarlett UK, Rutkowski MR, Rauwerdink AM, Fields J, Escovar-Fadul X, Baird J, Cubillos-Ruiz JR, Jacobs AC, Gonzalez JL, Weaver J, Fiering S, and Conejo-Garcia JR. 2012. J. Exp. Med. 209: 495-506. (Immunofluorescence microscopy - frozen tissue)Chen M, Felix K, and Wang J. 2011. J. Immunol. 187: 5684-5692. (in vitro blocking)Busman-Sahay K, Sargent E, Harton JA, and Drake JR. 2011. J. Immunol. 186:6710-6717. (Immunoprecipitation)Ohmura-Hoshino M, Matsuki Y, Aoki M, Goto E, Mito M, Uematsu M, Hakiuchi T, Hotta H, and Ishido S. 2006. J. Immunol. 177:341-354. (Immunofluorescence microscopy - frozen tissue, Immunoprecipitation)Li C, Siemasko K, Clark MR, and Song W. 2002. Int. Immunol. 14: 1179-1191. (Western Blot, Immunoelectron microscopy)

Limitations and Warranty

enQuire Bio's Mouse Anti-MHC Class II Monoclonal (I-A/I-E) is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

There are no reviews yet.

Be the first to review “Anti-MHC Class II Antibody (I-A/I-E) [M5/114.15.2]”

Your email address will not be published. Required fields are marked *

Share a Protocol (View Reviewed Protocols Below)

Protocol Title

Materials

Methods

Notes


  protocol submission gif

enQuire Bio, LLC   1825 Lawrence St, Ste 115   Denver, CO 80202
Phone: 1-855-344-1400 | enquire@enquirebio.com | Fax: 1-720-897-3730
Customer Service Available M-F 8AM-6PM MST or 24/7 Via Email
Scroll to top

We consume cookies ensure that we give you the best experience on our website. If you continue to use this site we will assume that you are happy with it. Dismiss

Anti-MHC Class II Antibody (I-A/I-E) [M5/114.15.2]https://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-QAB70-APC-100ug-anti-MHC-class-II-antigen-antibody-10-300x289.pnghttps://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-QAB70-APC7-100ug-anti-MHC-class-II-antigen-antibody-10-300x281.pnghttps://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-QAB70-F-100ug-anti-MHC-class-II-antigen-antibody-10-300x274.pnghttps://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-QAB70-PCP55-100ug-anti-MHC-class-II-antigen-antibody-10-300x265.pnghttps://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-QAB70-PE-100ug-anti-MHC-class-II-antigen-antibody-10-300x283.pnghttps://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-QAB70-QF710-100ug-anti-MHC-class-II-antigen-antibody-10-300x276.pnghttps://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-QAB70-V450-100ug-anti-MHC-class-II-antigen-antibody-10-300x270.pnghttps://enquirebio.com/wp-content/uploads/2017/11/enQuire-Bio-QAB70-APC-100ug-anti-MHC-class-II-antigen-antibody-9-300x289.pnghttps://enquirebio.com/wp-content/uploads/2017/11/enQuire-Bio-QAB70-APC7-100ug-anti-MHC-class-II-antigen-antibody-9-300x281.pnghttps://enquirebio.com/wp-content/uploads/2017/11/enQuire-Bio-QAB70-PCP55-100ug-anti-MHC-class-II-antigen-antibody-9-300x265.pnghttps://enquirebio.com/wp-content/uploads/2017/11/enQuire-Bio-QAB70-PE-100ug-anti-MHC-class-II-antigen-antibody-9-300x283.pnghttps://enquirebio.com/wp-content/uploads/2017/11/enQuire-Bio-QAB70-QF710-100ug-anti-MHC-class-II-antigen-antibody-9-300x276.pnghttps://enquirebio.com/wp-content/uploads/2017/11/enQuire-Bio-QAB70-V450-100ug-anti-MHC-class-II-antigen-antibody-9-300x270.png