Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Milk Fat Globule Monoclonal Antibody (MFG-6)
Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Milk Fat Globule Monoclonal Antibody (MFG-6)

Anti-Milk Fat Globule Antibody Clone MFG-06

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: 4240-MSM1
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 4240-MSM1-P1
Milk Fat Globule General Information
Alternate Names
Milk fat globule-EGF factor 8 protein, Mfge8, lactadherin
Molecular Weight
45kDa
Chromosomal Location
15q25
Curated Database and Bioinformatic Data
Gene SymbolMFGE8
Entrez Gene ID4240
Ensemble Gene IDENSG00000140545
RefSeq Protein Accession(s)NP_001108086, XP_016877695, NP_005919, NP_001297249, NP_001297248, NP_001297250
RefSeq mRNA Accession(s)NM_001310319, XM_017022206, NM_001114614, XR_931838, NM_001310321, NM_005928, NM_001310320
RefSeq Genomic Accession(s)NC_000015, NC_018926
UniProt ID(s)B3KTQ2, X6R3G6, Q08431, B4E396, F5GZN3
UniGene ID(s)B3KTQ2, X6R3G6, Q08431, B4E396, F5GZN3
HGNC ID(s)7036
Cosmic ID(s)MFGE8
KEGG Gene ID(s)hsa:4240
PharmGKB ID(s)PA30772
General Description of Milk Fat Globule.
Recognizes a protein of 40-45kDa, identified as human milk fat globule membrane protein (HMFG). HMFG is present on normal human breast epithelial cells, cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker, can also provide a tool to study the role of the cell surface in normal, neoplastic mammary development.

Human Anti-Milk Fat Globule Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 min at RT)
Clonality: Monoclonal
Anti-Milk Fat Globule Antibody Clone: MFG-06
Clone MFG-06 Host and Isotype: Mouse IgG1 kappa
Anti-Human Milk Fat Globule Positive Control Sample: Normal breast or breast carcinoma
Cellular Localization of Antibody Cell Surface & Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Human milk fat globule membrane preparation
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Milk Fat Globule Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-MFGE8 antibody staining is expected to be primarily localized to the cytosol.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Milk Fat Globule expression as measured by anti-Milk Fat Globule antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity +++ ++ +++ ++ +++ - +++ ++ +++ - - +++ +++ - +++ - +++ + + +++
MFGE8 Variability + +++ + ++ + ++ ++ ++ ++ + ++ + + ++ + ++ ++ ++ ++ +

Limitations and Warranty

enQuire Bio's Milk Fat Globule Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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