Human Anti-Milk Fat Globule Antibody Product Attributes
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 min at RT)
Clonality: Monoclonal
Anti-Milk Fat Globule Antibody Clone: MFG-06
Clone MFG-06 Host and Isotype: Mouse IgG1 kappa
Anti-Human Milk Fat Globule Positive Control Sample: Normal breast or breast carcinoma
Cellular Localization of Antibody Cell Surface & Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Human milk fat globule membrane preparation
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Milk Fat Globule Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-MFGE8 antibody staining is expected to be primarily localized to the cytosol.Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of Milk Fat Globule expression as measured by anti-Milk Fat Globule antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Signal Intensity | +++ | ++ | +++ | ++ | +++ | - | +++ | ++ | +++ | - | - | +++ | +++ | - | +++ | - | +++ | + | + | +++ |
MFGE8 Variability | + | +++ | + | ++ | + | ++ | ++ | ++ | ++ | + | ++ | + | + | ++ | + | ++ | ++ | ++ | ++ | + |
Limitations and Warranty
enQuire Bio's Milk Fat Globule Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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