Human Anti-Milk Fat Globule Antibody Product Attributes
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 min at RT)
Clonality: Monoclonal
Anti-Milk Fat Globule Antibody Clone: SPM291
Clone SPM291 Host and Isotype: Mouse IgG1 kappa
Anti-Human Milk Fat Globule Positive Control Sample: Normal breast or breast carcinoma
Cellular Localization of Antibody Cell Surface & Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Human milk fat globule membrane preparation
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Milk Fat Globule Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-MFGE8 antibody staining is expected to be primarily localized to the cytosol.Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of Milk Fat Globule expression as measured by anti-Milk Fat Globule antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.| Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal Intensity | +++ | ++ | +++ | ++ | +++ | - | +++ | ++ | +++ | - | - | +++ | +++ | - | +++ | - | +++ | + | + | +++ |
| MFGE8 Variability | + | +++ | + | ++ | + | ++ | ++ | ++ | ++ | + | ++ | + | + | ++ | + | ++ | ++ | ++ | ++ | + |
Limitations and Warranty
enQuire Bio's Milk Fat Globule Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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