Antibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
|Recommended Dilution (Conc)
||EDTA Buffer pH8.0
||60 min at Room Temperature
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Anti-MLH-1 Antibody Clone: G168-15
Host and Isotype: Mouse IgG1
Recommended Positive Control Sample: Tonsil, colon carcinoma
Cellular Localization of Antibody G168-15 Staining: Nuclear
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Lot specific. Plese contact tech support
Immunogen: Full length recombinant MLH.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
MLH-1 Information for Pathologists
Mismatch repair gene. Mutations associated with Lynch syndrome (hereditary non-polyposis colon cancer) and some cases of sporadic colon cancer. Terminology Also called MutL homolog 1 colon cancer nonpolyposis type 2 (NCBI-Gene), hMLH1. Pathophysiology
Common Uses By Pathologists:
Use immunostaining for MLH1, MSH2, PMS2 and MSH6 to screen for Lynch syndrome. Biopsy samples may be as reliable as resections (Am J Surg Pathol 2011;35:447). Use of only PMS2 and MSH6 antibodies may be as effective as using all 4 antibodies (Am J Surg Pathol 2009;33:1639, Mod Pathol 2011;24:1004). Cases with normal staining due to somatic hypermethylation can occur (Am J Surg Pathol 2011;35:1902). Microscopic (histologic) images
Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.