Western Blot  of (1) Recombinant MMP2 protein and (2) U87 Cell Lysate using MMP2 Monoclonal Antibody (MMP2/151).
Western Blot  of (1) Recombinant MMP2 protein and (2) U87 Cell Lysate using MMP2 Monoclonal Antibody (MMP2/151).

Anti-MMP2 / Collagenase Type IV A Antibody Clone MMP2/1501

$ 118.00$ 418.00
Please Select Product Options Below To View The Catalog Number.

SKU: 4313-MSM1
Species: Human
Tested Applications: Western Blot
Available Conjugates: Unconjugated
Isotype: Mouse IgG1
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
4313-MSM1-P0 Size: 20 ug, Tag: Unconjugated $ 118.00
4313-MSM1-P1 Size: 100 ug, Tag: Unconjugated $ 298.00
4313-MSM1-P2 Size: 200 ug, Tag: Unconjugated $ 418.00
Shipping Information
In Stock.
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
SKU: 4313-MSM1-P0
MMP2 / Collagenase Type IV A General Information
Alternate Names
matrix metalloproteinase-2, MMP-2, gelatinase, MMP2
Molecular Weight
72kDa (Pro) ;63kDa (cleaved)
Chromosomal Location
16q12.2
Curated Database and Bioinformatic Data
Gene SymbolMMP2
Entrez Gene ID4313
Ensemble Gene IDENSG00000087245
RefSeq Protein Accession(s)NP_004521, NP_001121363, NP_001289439, NP_001289437, NP_001289438
RefSeq mRNA Accession(s)NM_001302510, NM_004530, NM_001127891, NM_001302508, NM_001302509,
RefSeq Genomic Accession(s)NG_008989, NC_000016, NC_018927
UniProt ID(s)P08253, A0A024R6R4
UniGene ID(s)P08253, A0A024R6R4
HGNC ID(s)7166
Cosmic ID(s)MMP2
KEGG Gene ID(s)hsa:4313
PharmGKB ID(s)PA30877
General Description of MMP2 / Collagenase Type IV A.
This MAb recognizes a protein of 72kDa, which is identified as MMP2. The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, Fibronectin, Laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-2 (also designated type IV collagenase) cleaves collagen types IV,V, VII and X and gelatin type I. Activation of MMP-2 secretion requires the Ras signaling pathway.

Human Anti-MMP2 / Collagenase Type IV A Antibody Product Attributes

Species: Human
Tested Applications: Western Blot.
Clonality: Monoclonal
Anti-MMP2 / Collagenase Type IV A Antibody Clone: MMP2/1501
Clone MMP2/1501 Host and Isotype: Mouse IgG1
Anti-Human MMP2 / Collagenase Type IV A Positive Control Sample: U-138 MG or U-87 MG cells. Placenta
Cellular Localization of Antibody Cytoplasmic and Cell Surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA and 0.05% Azide
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human MMP2 protein fragment (aa444-575)
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

MMP2 / Collagenase Type IV A Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in MMP2 / Collagenase Type IV A antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in endothelial cells in the cerebral cortex and colon and trophoblastic cells in the placenta. More moderate antibody staining intensity was present in endothelial cells in the cerebral cortex and colon and trophoblastic cells in the placenta. Low, but measureable presence of MMP2 / Collagenase Type IV A could be seen in cells in the endometrial stroma in endometrium, cells in the glomeruli in kidney, cells in the red pulp in spleen, glandular cells in the cervix, uterine, fallopian tube, parathyroid gland and prostate, Leydig cells in the testis, myocytes in heart muscle and smooth muscle cells in the smooth muscle. We were unable to detect MMP2 / Collagenase Type IV A in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of MMP2 / Collagenase Type IV A expression as measured by anti-MMP2 / Collagenase Type IV A antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - - - - - - - - + - - - + - - -
MMP2 Variability + + + + + + ++ ++ ++ + + ++ ++ ++ ++ + ++ + ++ ++

Limitations and Warranty

enQuire Bio's MMP2 / Collagenase Type IV A Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-MMP2 / Collagenase Type IV A Antibody Clone MMP2/1501

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