Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with EMA Monoclonal Antibody (HMPV).

Anti-MUC1 / EMA / CD227 Antibody Clone HMPV

Name: Anti-MUC1 / EMA / CD227 Antibody Clone HMPV
Brand: enQuire Bio
Price: 429 USD
Availability: InStock

$ 429.00

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SKU: 4582-MSM14
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product Number	Description	Price
4582-MSM14-P1	Size: 100 ug, Tag: Unconjugated

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Datasheets and Documentation

Product Datasheet

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SKU: 4582-MSM14-P1

MUC1 / EMA / CD227 General Information
Alternate Names
Mucin 1, cell surface associated, MUC1, polymorphic epithelial mucin, PEM
Molecular Weight
265-400kDa
Chromosomal Location
1q22
Curated Database and Bioinformatic Data
Gene Symbol	MUC1
Entrez Gene ID	4582
Ensemble Gene ID	ENSG00000185499
RefSeq Protein Accession(s)	NP_001191214, NP_001191217, NP_001191219, NP_001191225, NP_001191216, NP_001191221, NP_001037857, NP_001037858, NP_001191224, NP_001191218, NP_001191222, NP_001191223, NP_002447, NP_001018016, NP_001018017, NP_001037855, NP_001037856, NP_001191215, NP_001191220, NP_001191226
RefSeq mRNA Accession(s)	NM_001044392, NM_001204292, NM_002456, NM_001018021, NM_182741, NM_001204286, NM_001204293, NM_001204294, NM_001044390, NM_001044393, NM_001044391, NM_001204291, NM_001204295, NM_001018016, NM_001204285, NM_001204287, NM_001204290, NM_001204297 NM_001018017, NM_001204288, NM_001204289, NM_001204296
RefSeq Genomic Accession(s)	NC_000001, NG_029383, NC_018912,
UniProt ID(s)	A0A0A0MRB3, A6ZIE6, A5YRU5, A6ZID7, A0A087X2A4, B6ECB3, A0A087X0L2, A0A0C4DGW3, Q7Z551, A6ZIE4, A6ZID6, A5YRV2, A5YRV0, Q7Z538, A5YRU7, P15941
UniGene ID(s)	A0A0A0MRB3, A6ZIE6, A5YRU5, A6ZID7, A0A087X2A4, B6ECB3, A0A087X0L2, A0A0C4DGW3, Q7Z551, A6ZIE4, A6ZID6, A5YRV2, A5YRV0, Q7Z538, A5YRU7, P15941
HGNC ID(s)	7508
Cosmic ID(s)	MUC1
KEGG Gene ID(s)	hsa:4582
PharmGKB ID(s)	PA31309
General Description of MUC1 / EMA / CD227.
This MAb recognizes full-length MUC1 in a glycosylation-independent manner, can bind to the fully glycosylated protein. The dominant epitope of this MAb is APDTR in the VNTR region. It reacts with the core peptide of the MUC1 protein, which is a member of a family of mucin glycoproteins that are characterized by high carbohydrate content, O-linked oligosaccharides, high molecular weight (>200kDa), an amino acid composition rich in serine, threonine, proline, glycine. The core protein contains a domain of 20 amino-acid tandem repeats that functions as multiple epitopes for the MAb. Incomplete glycosylation of some tumor-associated mucins may lead to variable unmasking of the multiple peptide epitopes leading to the observed differences in staining intensity between normal, malignant tissues. This MAb reacts with both normal, malignant epithelia of various tissues including breast, colon.

Human Anti-MUC1 / EMA / CD227 Antibody Product Attributes

Species: Human

Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).

Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-paraffin) (0.25-0.5ug of antibody/ml for 30 minutes at RT)

Clonality: Monoclonal

Anti-MUC1 / EMA / CD227 Antibody Clone: HMPV

Clone HMPV Host and Isotype: Mouse IgG1 kappa

Anti-Human MUC1 / EMA / CD227 Positive Control Sample: MCF-7 or MDA-231 cells. Breast, colon, ovarian, endometrial carcinoma.

Cellular Localization of Antibody Cytoplasmic, cell surface

Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.

Antibody Concentration: 200ug/ml

Antibody Purification Method:Protein A/G Purified

Immunogen: Human breast cancer cell line ZR-75 cells

Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.

MUC1 / EMA / CD227 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-MUC1 antibody staining is expected to be primarily localized to the plasma membrane and vesicles.

Observed Antibody Staining Data By Tissue Type:

Variations in MUC1 / EMA / CD227 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in respiratory epithelial cells in the bronchus, glandular cells in the cervix, uterine, colon, endometrium, fallopian tube and gallbladder, respiratory epithelial cells in the nasopharynx and glandular cells in the rectum and stomach. More moderate antibody staining intensity was present in respiratory epithelial cells in the bronchus, glandular cells in the cervix, uterine, colon, endometrium, fallopian tube and gallbladder, respiratory epithelial cells in the nasopharynx and glandular cells in the rectum and stomach. Low, but measureable presence of MUC1 / EMA / CD227 could be seen inglandular cells in the adrenal gland, hematopoietic cells in the bone marrow, myoepithelial cells in the breast, squamous epithelial cells in the cervix, uterine, macrophages in lung, non-germinal center cells in the lymph node, squamous epithelial cells in the oral mucosa, glandular cells in the prostate and salivary gland, fibroblasts in skin, keratinocytes in skin, Langerhans in skin, melanocytes in skin, epidermal cells in the skin, cells in the seminiferous ducts in testis, Leydig cells in the testis and squamous epithelial cells in the tonsil. We were unable to detect MUC1 / EMA / CD227 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of MUC1 / EMA / CD227 expression as measured by anti-MUC1 / EMA / CD227 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type	breast cancer	carcinoid	cervical cancer	colorectal cancer	endometrial cancer	glioma	head and neck cancer	liver cancer	lung cancer	lymphoma	melanoma	ovarian cancer	pancreatic cancer	prostate cancer	renal cancer	skin cancer	stomach cancer	testicular cancer	thyroid cancer	urothelial cancer
Signal Intensity	+++	-	+++	++	+++	-	+++	-	+++	-	-	+++	+++	-	+++	-	+++	-	++	++
MUC1 Variability	+	++	+	++	+	+	++	++	++	+	+	+	++	+	++	++	+	++	++	++

Limitations and Warranty

enQuire Bio's MUC1 / EMA / CD227 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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