Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with MUC-1 / EMA Monoclonal Antibody (SPM533).
Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with MUC-1 / EMA Monoclonal Antibody (SPM533).

Anti-MUC1 / EMA / CD227 Antibody Clone SPM533

$ 429.00
Please Select Product Options Below To View The Catalog Number.

SKU: 4582-MSM2X
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2a kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
4582-MSM2X-P1 Size: 100 ug, Tag: Unconjugated
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In Stock.
Flat Rate Shipping Anywhere in the US: $45
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SKU: 4582-MSM2X-P1
MUC1 / EMA / CD227 General Information
Alternate Names
Mucin 1, cell surface associated, MUC1, polymorphic epithelial mucin, PEM
Molecular Weight
265-400kDa
Chromosomal Location
1q22
Curated Database and Bioinformatic Data
Gene SymbolMUC1
Entrez Gene ID4582
Ensemble Gene IDENSG00000185499
RefSeq Protein Accession(s)NP_001191214, NP_001191217, NP_001191219, NP_001191225, NP_001191216, NP_001191221, NP_001037857, NP_001037858, NP_001191224, NP_001191218, NP_001191222, NP_001191223, NP_002447, NP_001018016, NP_001018017, NP_001037855, NP_001037856, NP_001191215, NP_001191220, NP_001191226
RefSeq mRNA Accession(s)NM_001044392, NM_001204292, NM_002456, NM_001018021, NM_182741, NM_001204286, NM_001204293, NM_001204294, NM_001044390, NM_001044393, NM_001044391, NM_001204291, NM_001204295, NM_001018016, NM_001204285, NM_001204287, NM_001204290, NM_001204297 NM_001018017, NM_001204288, NM_001204289, NM_001204296
RefSeq Genomic Accession(s)NC_000001, NG_029383, NC_018912,
UniProt ID(s)A0A0A0MRB3, A6ZIE6, A5YRU5, A6ZID7, A0A087X2A4, B6ECB3, A0A087X0L2, A0A0C4DGW3, Q7Z551, A6ZIE4, A6ZID6, A5YRV2, A5YRV0, Q7Z538, A5YRU7, P15941
UniGene ID(s)A0A0A0MRB3, A6ZIE6, A5YRU5, A6ZID7, A0A087X2A4, B6ECB3, A0A087X0L2, A0A0C4DGW3, Q7Z551, A6ZIE4, A6ZID6, A5YRV2, A5YRV0, Q7Z538, A5YRU7, P15941
HGNC ID(s)7508
Cosmic ID(s)MUC1
KEGG Gene ID(s)hsa:4582
PharmGKB ID(s)PA31309
General Description of MUC1 / EMA / CD227.
In Western Blot, it recognizes proteins in MW range of 265-400kDa, identified as different glycoforms of EMA. This protein is proteolytically cleaved into alpha, beta subunits that form a heterodimeric complex. The N-terminal alpha subunit, the C-terminal beta subunit. . The alpha subunit has cell adhesive properties. It can act both as an adhesion, an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial, enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylations, protein-protein interactions. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinomas. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

Human Anti-MUC1 / EMA / CD227 Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.1-0.2ug of antibody/ml for 30 min at RT)
Clonality: Monoclonal
Anti-MUC1 / EMA / CD227 Antibody Clone: SPM533
Clone SPM533 Host and Isotype: Mouse IgG2a kappa
Anti-Human MUC1 / EMA / CD227 Positive Control Sample: Breast or colon carcinoma.
Cellular Localization of Antibody Cytoplasmic & cell surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Delipidated extract of human milk fat globule membranes
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

MUC1 / EMA / CD227 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-MUC1 antibody staining is expected to be primarily localized to the plasma membrane and vesicles.

Observed Antibody Staining Data By Tissue Type:

Variations in MUC1 / EMA / CD227 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in respiratory epithelial cells in the bronchus, glandular cells in the cervix, uterine, colon, endometrium, fallopian tube and gallbladder, respiratory epithelial cells in the nasopharynx and glandular cells in the rectum and stomach. More moderate antibody staining intensity was present in respiratory epithelial cells in the bronchus, glandular cells in the cervix, uterine, colon, endometrium, fallopian tube and gallbladder, respiratory epithelial cells in the nasopharynx and glandular cells in the rectum and stomach. Low, but measureable presence of MUC1 / EMA / CD227 could be seen inglandular cells in the adrenal gland, hematopoietic cells in the bone marrow, myoepithelial cells in the breast, squamous epithelial cells in the cervix, uterine, macrophages in lung, non-germinal center cells in the lymph node, squamous epithelial cells in the oral mucosa, glandular cells in the prostate and salivary gland, fibroblasts in skin, keratinocytes in skin, Langerhans in skin, melanocytes in skin, epidermal cells in the skin, cells in the seminiferous ducts in testis, Leydig cells in the testis and squamous epithelial cells in the tonsil. We were unable to detect MUC1 / EMA / CD227 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of MUC1 / EMA / CD227 expression as measured by anti-MUC1 / EMA / CD227 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity +++ - +++ ++ +++ - +++ - +++ - - +++ +++ - +++ - +++ - ++ ++
MUC1 Variability + ++ + ++ + + ++ ++ ++ + + + ++ + ++ ++ + ++ ++ ++

Limitations and Warranty

enQuire Bio's MUC1 / EMA / CD227 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-MUC1 / EMA / CD227 Antibody Clone SPM533

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