Human Anti-MUC18 / CD146 / MCAM Antibody Product Attributes
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1.0ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1.0ug of antibody/ml for 30 minutes at RT)
Anti-MUC18 / CD146 / MCAM Antibody Clone: SPM620
Clone SPM620 Host and Isotype: Mouse IgG1 kappa
Anti-Human MUC18 / CD146 / MCAM Positive Control Sample: A-375, HUV-EC or HeLa Cells. Tonsil or Melanoma.
Cellular Localization of Antibody
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human MCAM protein
Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.
MUC18 / CD146 / MCAM Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:Anti-MCAM antibody staining is expected to be primarily localized to the plasma membrane.
Observed Antibody Staining Data By Tissue Type:Variations in MUC18 / CD146 / MCAM antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in adipocytes in mesenchymal tissue, endothelial cells in the cerebral cortex and colon, fibroblasts in mesenchymal tissue, neuropil in cerebral cortex and peripheral nerve in mesenchymal tissue. More moderate antibody staining intensity was present in adipocytes in mesenchymal tissue, endothelial cells in the cerebral cortex and colon, fibroblasts in mesenchymal tissue, neuropil in cerebral cortex and peripheral nerve in mesenchymal tissue. Low, but measureable presence of MUC18 / CD146 / MCAM could be seen infibroblasts in skin, glial cells in the hippocampus, myocytes in heart muscle, neuronal cells in the cerebral cortex, Purkinje cells in the cerebellum and smooth muscle cells in the smooth muscle. We were unable to detect MUC18 / CD146 / MCAM in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Observed Antibody Staining Data By Tissue Disease Status:Tissues from cancer patients, for instance, have their own distinct pattern of MUC18 / CD146 / MCAM expression as measured by anti-MUC18 / CD146 / MCAM antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
|Sample Type||breast cancer||carcinoid||cervical cancer||colorectal cancer||endometrial cancer||glioma||head and neck cancer||liver cancer||lung cancer||lymphoma||melanoma||ovarian cancer||pancreatic cancer||prostate cancer||renal cancer||skin cancer||stomach cancer||testicular cancer||thyroid cancer||urothelial cancer|
Limitations and Warranty
enQuire Bio's MUC18 / CD146 / MCAM Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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