Formalin-fixed, paraffin-embedded human Melanoma stained with MCAM Monoclonal Antibody (SPM62)
Formalin-fixed, paraffin-embedded human Melanoma stained with MCAM Monoclonal Antibody (SPM62)

Anti-MUC18 / CD146 / MCAM Antibody Clone SPM620

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: 4162-MSM2X
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 4162-MSM2X-P1
MUC18 / CD146 / MCAM General Information
Alternate Names
CD146, cluster of differentiation 146, melanoma cell adhesion molecule, MCAM, cell surface glycoprotein MUC18
Molecular Weight
130kDa
Chromosomal Location
11q23.3
Curated Database and Bioinformatic Data
Gene SymbolMCAM
Entrez Gene ID4162
Ensemble Gene IDENSG00000076706
RefSeq Protein Accession(s)XP_016873248, NP_006491, XP_016873249, XP_016873250, XP_016873251
RefSeq mRNA Accession(s)XM_017017760, NM_006500, XM_017017759, XM_017017762, XM_017017761
RefSeq Genomic Accession(s)NC_018922, NC_000011
UniProt ID(s)P43121, A0A024R3I5
UniGene ID(s)P43121, A0A024R3I5
HGNC ID(s)6934
Cosmic ID(s)MCAM
KEGG Gene ID(s)hsa:4162
PharmGKB ID(s)PA30678
General Description of MUC18 / CD146 / MCAM.
The human Mel-CAM gene maps to chromosome 11q23, encodes a trans-membrane glycoprotein, also designated MCAM, MUC 18 or CD146, that belongs to the immunoglobulin superfamily, functions as a Ca2+-independent cell adhesion molecule. Mel-CAM expression is restricted to advanced primary, metastatic melanomas, to cell lines of the neuroectodermal lineage, but not normal melanocytes. Mel-CAM is found on 80% of advanced primary human melanomas, correlates well with development of metastatic disease.

Human Anti-MUC18 / CD146 / MCAM Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1.0ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1.0ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-MUC18 / CD146 / MCAM Antibody Clone: SPM620
Clone SPM620 Host and Isotype: Mouse IgG1 kappa
Anti-Human MUC18 / CD146 / MCAM Positive Control Sample: A-375, HUV-EC or HeLa Cells. Tonsil or Melanoma.
Cellular Localization of Antibody Cell surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human MCAM protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

MUC18 / CD146 / MCAM Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-MCAM antibody staining is expected to be primarily localized to the plasma membrane.

Observed Antibody Staining Data By Tissue Type:

Variations in MUC18 / CD146 / MCAM antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in adipocytes in mesenchymal tissue, endothelial cells in the cerebral cortex and colon, fibroblasts in mesenchymal tissue, neuropil in cerebral cortex and peripheral nerve in mesenchymal tissue. More moderate antibody staining intensity was present in adipocytes in mesenchymal tissue, endothelial cells in the cerebral cortex and colon, fibroblasts in mesenchymal tissue, neuropil in cerebral cortex and peripheral nerve in mesenchymal tissue. Low, but measureable presence of MUC18 / CD146 / MCAM could be seen infibroblasts in skin, glial cells in the hippocampus, myocytes in heart muscle, neuronal cells in the cerebral cortex, Purkinje cells in the cerebellum and smooth muscle cells in the smooth muscle. We were unable to detect MUC18 / CD146 / MCAM in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of MUC18 / CD146 / MCAM expression as measured by anti-MUC18 / CD146 / MCAM antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - + - + - + - ++ - - - - - - + - -
MCAM Variability ++ ++ ++ + ++ ++ ++ ++ ++ + ++ + ++ + + ++ + ++ ++ +

Limitations and Warranty

enQuire Bio's MUC18 / CD146 / MCAM Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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