Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC3 Monoclonal Antibody (M3.1).
Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC3 Monoclonal Antibody (M3.1).Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MUC3 Monoclonal Antibody (M3.1).

Anti-MUC3 Antibody Clone M3.1

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: 4584-MSM1
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2a kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
4584-MSM1-P1 Size: 100 ug, Tag: Unconjugated
4584-MSM1-P1 Size: 100 ug
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In Stock.
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SKU: 4584-MSM1-P1
MUC3 General Information
Alternate Names
Mucin 3A, MUC3A
Molecular Weight
1100kDa
Chromosomal Location
7q22.1
Curated Database and Bioinformatic Data
Gene SymbolMUC3A
Entrez Gene ID4584, 57876
Ensemble Gene IDENSG00000169894
RefSeq Protein Accession(s)XP_011514549, XP_011514551, XP_016867720, XP_011514552, XP_011514548, NP_005951
RefSeq mRNA Accession(s)XM_011516246, XM_011516247, XM_011516250, XM_017012231, XM_011516249, NM_005960
RefSeq Genomic Accession(s)NC_018918, NC_000007
UniProt ID(s)Q9UN95, Q02505, Q9H3Q6
UniGene ID(s)Q9UN95, Q02505, Q9H3Q6
HGNC ID(s)7513, 13384
Cosmic ID(s)MUC3A, MUC3B
KEGG Gene ID(s)hsa:4584
General Description of MUC3.
It recognizes a protein of HMW, identified as mucin 3 glycoprotein (MUC3). Its epitope localizes between aa SITTTE. This MAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC2. MUC3 is distributed in colon, rectum,, is also present to a lesser extent in breast, lung, salivary gland tissues. The Mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs) encoded by 60 base pairs (Mucin 1), 69 base pairs (Mucin 2), 51 base pairs (Mucin 3). The number of repeats is highly polymorphic, varies among different alleles. Mucin 1 proteins are expressed as type I membrane proteins in addition to secreted forms. Mucin 1 is aberrantly expressed in epithelial tumors including breast carcinomas. Mucin 2 coats the epithelia of the intestines, airways, is associated with colonic tumors. Mucin 3 is a major component of various mucus gels, is broadly expressed in normal, tumor cells.

Human Anti-MUC3 Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-MUC3 Antibody Clone: M3.1
Clone M3.1 Host and Isotype: Mouse IgG2a kappa
Anti-Human MUC3 Positive Control Sample: LS174T cells or colon carcinoma, normal colon tissue.
Cellular Localization of Antibody Cytoplasmic, cell surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: A synthetic peptide of 35 amino acids, SIB35 (C-HSTPSFTSSITTTETTSHSTPSFTSSITTTETTS), which contains two of the MUC3 tandem repeats, coupled to KLH.
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

MUC3 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of MUC3 expression as measured by anti-MUC3 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - + - + - - - + - - - - - + + - - - + -
MUC3A Variability + +++ ++ ++ ++ + + ++ + + ++ ++ + ++ ++ + + ++ ++ +

Limitations and Warranty

enQuire Bio's MUC3 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-MUC3 Antibody Clone M3.1

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