Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with MyoD1 Monoclonal Antibody (SPM427)
Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with MyoD1 Monoclonal Antibody (SPM427)

Anti-MyoD1 Antibody Clone SPM427

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: 4654-MSM1X
Species: Human, Mouse, Rat, and Chicken
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
4654-MSM1X-P1 Size: 100 ug, Tag: Unconjugated
Shipping Information
In Stock.
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Datasheets and Documentation
Product Datasheet
SKU: 4654-MSM1X-P1
MyoD1 General Information
Alternate Names
MyoD, MyoD1
Molecular Weight
45kDa
Chromosomal Location
11p15.1
Curated Database and Bioinformatic Data
Gene SymbolMYOD1
Entrez Gene ID4654
Ensemble Gene IDENSG00000129152
RefSeq Protein Accession(s)NP_002469
RefSeq mRNA Accession(s)NM_002478
RefSeq Genomic Accession(s)NC_018922, NC_000011
UniProt ID(s)P15172
UniGene ID(s)P15172
HGNC ID(s)7611
Cosmic ID(s)MYOD1
KEGG Gene ID(s)hsa:4654
PharmGKB ID(s)PA31416
General Description of MyoD1.
Recognizes a phosphor-protein of 45kDa, identified as MyoD1. The epitope of this MAb maps between amino acid 180-189 in the C-terminal of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma, a subset of Wilms tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium, also in rhabdomyosarcomas, neuroblastomas, Ewings sarcomas, alveolar soft part sarcomas.

Human, Mouse, Rat, and Chicken Anti-MyoD1 Antibody Product Attributes

Species: Human, Mouse, Rat, and Chicken
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-MyoD1 Antibody Clone: SPM427
Clone SPM427 Host and Isotype: Mouse IgG1 kappa
Anti-Human, Mouse, Rat, and Chicken MyoD1 Positive Control Sample: Rhabdomyosarcoma
Cellular Localization of Antibody Nuclear. Only nuclear staining should be considered as evidence of skeletal muscle differentiation.
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant mouse MyoD1 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

MyoD1 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-MYOD1 antibody staining is expected to be primarily localized to the nuclear bodies and nucleoplasm.

Limitations and Warranty

enQuire Bio's MyoD1 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-MyoD1 Antibody Clone SPM427

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