Antibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
|Recommended Dilution (Conc)||1:25-1:50|
|Pretreatment||Citrate Buffer pH 6.0|
|Incubation Parameters||30 min at Room Temperature|
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Anti-MyoD1 Antibody Clone: 5.2F
Host and Isotype: Mouse IgG2a
Recommended Positive Control Sample: Rhabdomyosarcoma
Cellular Localization of Antibody 5.2F Staining: Nuclear
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: BALB/C mice were injected with a recombinant mouse MyoD1 protein.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
MyoD1 Information for Pathologists
Summary:Myogenic transcriptional regulatory protein expressed early in skeletal muscle differentiation. Sensitive but not specific for rhabdomyosarcoma due to cytoplasmic and non-specific background staining (Am J Surg Pathol 2001;25:1150). Stronger staining in alveolar vs. embryonal rhabdomyosarcomasar germ cell tumors (also other cells as indicated below). Interpretation Nuclear immunostaining.
Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
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