Immunohistochemical staining of MyoD1  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

MyoD1 [5.2F] Antibody (cGMP).

$ 135.00$ 645.00
Please Select Product Options Below To View The Catalog Number.

Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Mouse IgG2a

Product NumberDescriptionPrice
QIVD155-0.1ml Size: 0.1 ml, Format: Concentrate $ 158.00
QIVD155-0.5ml Size: 0.5 ml, Format: Concentrate $ 388.00
QIVD155-1ml Size: 1 ml, Format: Concentrate $ 648.00
QIVD155-6ml Size: 6 ml, Format: Predilute $ 288.00
QIVD155-25ml Size: 25 ml, Format: Predilute $ 728.00
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Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

Expiration Date:

Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

Manufacture Date:


Bioactivity (test results eg. IU/ml):

SKU: QIVD155-0.1ml
MyoD1 General Information
Alternate Names
Molecular Weight
34.5 kDa
Chromosomal Location
p15.1 [chr: 11] [chr_start: 17719571] [chr_end: 17722136] [strand: 1]
Curated Database and Bioinformatic Data
Gene SymbolMYOD1
Entrez Gene ID4654
RefSeq Protein Accession(s)NP_002469
RefSeq mRNA Accession(s)NM_002478
RefSeq Genomic Accession(s)NC_000011
UniProt ID(s)P15172
PharmGKB ID(s)PA31416
KEGG Gene ID(s)hsa:4654
General Description of MyoD1 .
This antibody is specific to a 45 kDa protein, which is identified as MyoD1. This antibody is specific to an epitope of amino acid 3-56 in the N-terminus of mouse MyoD1. This antibody does not react with myogenin, Myf5 or Myf6. MyoD1 stains the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue but is expressed strongly in the tumor cell nuclei of rhabdomyosarcomas.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:25-1:50
Pretreatment Citrate Buffer pH 6.0
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-MyoD1 Antibody Clone: 5.2F
Host and Isotype: Mouse IgG2a
Recommended Positive Control Sample: Rhabdomyosarcoma
Cellular Localization of Antibody 5.2F Staining: Nuclear
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: BALB/C mice were injected with a recombinant mouse MyoD1 protein.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

MyoD1 Information for Pathologists


Myogenic transcriptional regulatory protein expressed early in skeletal muscle differentiation. Sensitive but not specific for rhabdomyosarcoma due to cytoplasmic and non-specific background staining (Am J Surg Pathol 2001;25:1150). Stronger staining in alveolar vs. embryonal rhabdomyosarcomasar germ cell tumors (also other cells as indicated below). Interpretation Nuclear immunostaining.

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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MyoD1 [5.2F] Antibody (cGMP).