Human and Mouse Anti-N-Cadherin / Cadherin-2 / CD325 Antibody Product Attributes
Species: Human and Mouse
Tested Applications: Immunohistochemistry (IHC).
Clonality: Monoclonal
Anti-N-Cadherin / Cadherin-2 / CD325 Antibody Clone: CDH2/1573
Clone CDH2/1573 Host and Isotype: Mouse IgG1 kappa
Anti-Human and Mouse N-Cadherin / Cadherin-2 / CD325 Positive Control Sample: HeLa or HUVEC cells. Heart, Pancreas or Cerebral Cortex
Cellular Localization of Antibody Cell surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA and 0.05% Azide
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human CDH2 intracellular domain
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
N-Cadherin / Cadherin-2 / CD325 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-CDH2 antibody staining is expected to be primarily localized to the cell junctions and plasma membrane.Observed Antibody Staining Data By Tissue Type:
Variations in N-Cadherin / Cadherin-2 / CD325 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in bile duct cells in the liver, cells in the seminiferous ducts in testis, cells in the tubules in kidney, glandular cells in the adrenal gland and fallopian tube, hepatocytes in liver, myocytes in heart muscle and neuropil in cerebral cortex. More moderate antibody staining intensity was present in bile duct cells in the liver, cells in the seminiferous ducts in testis, cells in the tubules in kidney, glandular cells in the adrenal gland and fallopian tube, hepatocytes in liver, myocytes in heart muscle and neuropil in cerebral cortex. Low, but measureable presence of N-Cadherin / Cadherin-2 / CD325 could be seen in cells in the granular layer in cerebellum, glandular cells in the endometrium, stomach and thyroid gland and islets of Langerhans in pancreas. We were unable to detect N-Cadherin / Cadherin-2 / CD325 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of N-Cadherin / Cadherin-2 / CD325 expression as measured by anti-N-Cadherin / Cadherin-2 / CD325 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Signal Intensity | - | ++ | - | - | + | - | - | +++ | - | - | - | - | - | - | ++ | - | - | + | ++ | - |
CDH2 Variability | + | ++ | + | + | +++ | + | + | ++ | ++ | + | ++ | ++ | ++ | + | ++ | + | + | +++ | ++ | + |
Limitations and Warranty
enQuire Bio's N-Cadherin / Cadherin-2 / CD325 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
There are no reviews yet.