Staining by anti-N-Cadherin / Cadherin-2 / CD325 Antibody 1

Anti-N-Cadherin / Cadherin-2 / CD325 Antibody Clone CDH2/1573

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SKU: 1000-MSM3
Species: Human and Mouse
Tested Applications: Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 1000-MSM3-P0
N-Cadherin / Cadherin-2 / CD325 General Information
Alternate Names
N-cadherin Cadherin-2, neural cadherin, NCAD, CDH2
Molecular Weight
130-140kDa
Chromosomal Location
18q11.2
Curated Database and Bioinformatic Data
Gene SymbolCDH2
Entrez Gene ID1000
Ensemble Gene IDENSG00000170558
RefSeq Protein Accession(s)NP_001783, XP_016881003, XP_011524090, NP_001295105
RefSeq mRNA Accession(s)NM_001308176, XM_011525788, NM_001792
RefSeq Genomic Accession(s)NC_000018, NG_011959, NC_018929
UniProt ID(s)P19022
UniGene ID(s)Hs464829, Hs606106
HGNC ID(s)1759
Cosmic ID(s)CDH2
KEGG Gene ID(s)hsa:1000
PharmGKB ID(s)PA26293
General Description of N-Cadherin / Cadherin-2 / CD325.
Recognizes a protein of ~140kDa, identified as N-Cadherin (NCAD), also known as CD325. N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. Expression of N-cadherin has been reported on a variety of normal tissues including neuronal, endothelial and muscle cells, and a subpopulation of early hematopoietic progenitor cells. Results aid in the classification of malignant non-carcinomatous neoplasms including mesotheliomas, chordomas, synovial sarcomas, malignant melanomas, epithelioid sarcomas, epithelioid angiosarcomas, clear cell sarcomas as well as serous and endometrioid tumors of the ovary have been demonstrated to be N-cadherin positive, whereas mucinous tumors are negative. Other N-cadherin-positive neoplasms include renal cell carcinomas and some variant breast tumors, including medullary breast carcinomas and sarcomatoid metaplastic breast carcinomas.

Human and Mouse Anti-N-Cadherin / Cadherin-2 / CD325 Antibody Product Attributes

Species: Human and Mouse
Tested Applications: Immunohistochemistry (IHC).
Clonality: Monoclonal
Anti-N-Cadherin / Cadherin-2 / CD325 Antibody Clone: CDH2/1573
Clone CDH2/1573 Host and Isotype: Mouse IgG1 kappa
Anti-Human and Mouse N-Cadherin / Cadherin-2 / CD325 Positive Control Sample: HeLa or HUVEC cells. Heart, Pancreas or Cerebral Cortex
Cellular Localization of Antibody Cell surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA and 0.05% Azide
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human CDH2 intracellular domain
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

N-Cadherin / Cadherin-2 / CD325 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-CDH2 antibody staining is expected to be primarily localized to the cell junctions and plasma membrane.

Observed Antibody Staining Data By Tissue Type:

Variations in N-Cadherin / Cadherin-2 / CD325 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in bile duct cells in the liver, cells in the seminiferous ducts in testis, cells in the tubules in kidney, glandular cells in the adrenal gland and fallopian tube, hepatocytes in liver, myocytes in heart muscle and neuropil in cerebral cortex. More moderate antibody staining intensity was present in bile duct cells in the liver, cells in the seminiferous ducts in testis, cells in the tubules in kidney, glandular cells in the adrenal gland and fallopian tube, hepatocytes in liver, myocytes in heart muscle and neuropil in cerebral cortex. Low, but measureable presence of N-Cadherin / Cadherin-2 / CD325 could be seen in cells in the granular layer in cerebellum, glandular cells in the endometrium, stomach and thyroid gland and islets of Langerhans in pancreas. We were unable to detect N-Cadherin / Cadherin-2 / CD325 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of N-Cadherin / Cadherin-2 / CD325 expression as measured by anti-N-Cadherin / Cadherin-2 / CD325 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - ++ - - + - - +++ - - - - - - ++ - - + ++ -
CDH2 Variability + ++ + + +++ + + ++ ++ + ++ ++ ++ + ++ + + +++ ++ +

Limitations and Warranty

enQuire Bio's N-Cadherin / Cadherin-2 / CD325 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-N-Cadherin / Cadherin-2 / CD325 Antibody Clone CDH2/1573