Western Blot of K562 and HEK293 Cell Lysates Using Napsin-A Monoclonal Antibody (NAPSA/1239)

Anti-Napsin A Antibody Clone NAPSA/1239

$ 429.00
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SKU: 9476-MSM3
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
9476-MSM3-P1 Size: 100 ug, Tag: Unconjugated
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SKU: 9476-MSM3-P1
Napsin A General Information
Alternate Names
Napsin-A, NAPSA
Molecular Weight
Chromosomal Location
Curated Database and Bioinformatic Data
Gene SymbolNAPSA
Entrez Gene ID9476
Ensemble Gene IDENSG00000131400
RefSeq Protein Accession(s)XP_011525842, NP_004842, XP_016883001
RefSeq mRNA Accession(s)NM_004851, XM_011527540, XM_017027512
RefSeq Genomic Accession(s)NC_018930, NC_000019
UniProt ID(s)O96009
UniGene ID(s)O96009
HGNC ID(s)13395
Cosmic ID(s)NAPSA
KEGG Gene ID(s)hsa:9476
PharmGKB ID(s)PA134891814
General Description of Napsin A.
Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B.There are two closely related napsins, napsin A, napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung, kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes, in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.

Human Anti-Napsin A Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Western Blot (1-2ug of antibody/ml for 60 minutes at RT), Immunohistochemistry (IHC) (Formalin-fixed) (1-2ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Napsin A Antibody Clone: NAPSA/1239
Clone NAPSA/1239 Host and Isotype: Mouse IgG kappa
Anti-Human Napsin A Positive Control Sample: Lung adenocarcinoma
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant fragment of human Napsin-A protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Napsin A Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in Napsin A antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the tubules in kidney and macrophages in lung. More moderate antibody staining intensity was present in cells in the tubules in kidney and macrophages in lung. Low, but measureable presence of Napsin A could be seen in. We were unable to detect Napsin A in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Napsin A expression as measured by anti-Napsin A antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - - - - ++ - - - - - - - - - - -
NAPSA Variability + + + + + + ++ + ++ + + + + + ++ + + + + +

Limitations and Warranty

enQuire Bio's Napsin A Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Napsin A Antibody Clone NAPSA/1239

https://enquirebio.com/wp-content/uploads/2019/01/Napsin A IHC human Lung Adenocarcinoma (NAPSA 1239)-300x225.jpghttps://enquirebio.com/wp-content/uploads/2019/01/NAPSA1239 WB K562 HE293-235x300.jpghttps://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-9476-MSM3-P1-anti-Napsin-A-antibody-300x225.jpeg