Immunohistochemical staining of Napsin A  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

Napsin A [KCG1.1] Antibody (cGMP).

$ 135.00$ 565.00
Please Select Product Options Below To View The Catalog Number.

SKU: QIVD157
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Mouse IgG1

Product NumberDescriptionPrice
QIVD157-0.1ml Size: 0.1 ml, Format: Concentrate $ 135.00
QIVD157-0.5ml Size: 0.5 ml, Format: Concentrate $ 355.00
QIVD157-1ml Size: 1 ml, Format: Concentrate $ 565.00
QIVD157-6ml Size: 6 ml, Format: Predilute $ 175.00
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Datasheets and Documentation
Product Datasheet
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SKU: QIVD157-0.1ml
Napsin A General Information
Alternate Names
Molecular Weight
45.4 kDa
Chromosomal Location
q13.33 [chr: 19] [chr_start: 50358477] [chr_end: 50365830] [strand: -1]
Curated Database and Bioinformatic Data
Gene SymbolNAPSA
Entrez Gene ID9476
RefSeq Protein Accession(s)NP_004842; XP_011525842
RefSeq mRNA Accession(s)NM_004851; XM_011527540; XM_017027512
RefSeq Genomic Accession(s)NC_000019
UniProt ID(s)O96009
PharmGKB ID(s)PA134891814
KEGG Gene ID(s)hsa:9476
General Description of Napsin A .
Napsin is found in two isoforms, A and B, with highly homologous gene sequences. Napsin A has a molecular weight of 35.0 kDa and is also known as TA02. Napsin A is an aspartic proteinase which is expressed in the lung and involved in processing surfactant protein B (SP-B). It is also expressed in the kidney. This antibody may be a useful tool as a tumor marker for primary lung adenocarcinoma. Napsin expression correlates with the differentiation grade of lung adenocarcinoma.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:50-1:100
Pretreatment Citrate Buffer pH 6.0
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-Napsin A Antibody Clone: KCG1.1
Host and Isotype: Mouse IgG1
Recommended Positive Control Sample: Lung adenocarcinoma
Cellular Localization of Antibody KCG1.1 Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: BALB/C mice were injected with synthetic peptide from N terminus of human napsin A
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

Napsin A Information for Pathologists

Summary:

A novel aspartic proteinase of the pepsin family involved in the maturation of surfactant protein B. Found primarily in lung and kidney. Lack of NapsinA expression in tumor cells may be poor prognostic marker in pulmonary adenocarcinoma (Lung Cancer 2012;77:156). Uses by pathologists Useful individually (Am J Surg Pathol 2012;36:396) or as part of panel (Arch Pathol Lab Med 2012;136:155, Appl Immunohistochem Mol Morphol 2012;20:350) to distinguish lung adenocarcinoma (positive) from squamous cell carcinoma (negative in tumor cells but positive in hyperplastic type II pneumocytes and intra-alveolar macrophages entrapped within tumor cells).

Common Uses By Pathologists:

Useful individually (Am J Surg Pathol 2012;36:396) or as part of panel (Arch Pathol Lab Med 2012;136:155, Appl Immunohistochem Mol Morphol 2012;20:350) to distinguish lung adenocarcinoma (positive) from squamous cell carcinoma (negative in tumor cells but positive in hyperplastic type II pneumocytes and intra-alveolar macrophages entrapped within tumor cells). Useful as part of panel to classify poorly differentiated non small cell lung carcinoma on small biopsies (Am J Surg Pathol 2011;35:15), fine needle aspirates (Cytojournal 2012;9:10) or bronchial brushings (Cancer Cytopathol 2011;119:335). Superior to TTF1 in distinguishing primary lung adenocarcinoma from other carcinomas (except kidney), particularly primary lung small cell carcinoma and primary thyroid carcinoma (Arch Pathol Lab Med 2012;136:163). Superior to TTF1 in distinguishing metastatic pulmonary (positive) from nonpulmonary (negative) adenocarcinoma in cell blocks prepared from malignant pleural effusions (Acta Cytol 2011;55:266) or from fine needle aspirates (Cancer Cytopathol 2011;119:127). May help identify metastatic disease with unknown primary as originating in lung (Case Rep Oncol 2011;4:564).

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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Napsin A [KCG1.1] Antibody (cGMP).