Human, Rat, Pig, Cow, and Chicken Anti-Neurofilament Antibody Product Attributes
Species: Human, Rat, Pig, Cow, and Chicken
Tested Applications: Immunofluorescence, Flow Cytometry, Immunohistochemistry (IHC).
Application Notes: Immunofluorescence (1-2ug of antibody/ml), Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.25-0.5ug of antibody/ml for 30 minutes at RT)
Anti-Neurofilament Antibody Clone: NR-4
Clone NR-4 Host and Isotype: Mouse IgG1
Anti-Human, Rat, Pig, Cow, and Chicken Neurofilament Positive Control Sample: Brain, Neuroblastoma
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Crude neurofilament preparation from porcine spinal cord
Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.
Neurofilament Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-NEFL antibody staining is expected to be primarily localized to the intermediate filaments and nuclear interchromatin granular clusters. There is variability in either the signal strength or the localization of signal in intermediate filaments from cell to cell.
Observed Antibody Staining Data By Tissue Type:
Variations in Neurofilament antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in neuronal cells in the caudate nucleus, cerebral cortex. More moderate antibody staining intensity was present in neuronal cells in the caudate nucleus, cerebral cortex. Low, but measureable presence of Neurofilament could be seen in cells in the granular layer in cerebellum. We were unable to detect Neurofilament in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Limitations and Warranty
enQuire Bio's Neurofilament Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.