Formalin-fixed, paraffin-embedded Rat Cerebellum stained with Neurofilament Monoclonal Antibody (RT-97 + NR-4).
Formalin-fixed, paraffin-embedded Rat Cerebellum stained with Neurofilament Monoclonal Antibody (RT-97 + NR-4).

Anti-Neurofilament Antibody Clone RT-97 + NR-4

$ 429.00
Please Select Product Options Below To View The Catalog Number.

SKU: MSM2-1201
Species: Human, Mouse, Rat, Chicken, and Pig
Tested Applications: Flow Cytometry, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
MSM2-1201-P1 Size: 100 ug, Tag: Unconjugated
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In Stock.
Flat Rate Shipping Anywhere in the US: $45
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SKU: MSM2-1201-P1
Neurofilament General Information
Alternate Names
NEFH, Neurofilament H, Neurofilament Heavy Polypeptide 2kDa, Neurofilament Triplet H Protein, NF-H, NF2. 68kDa neurofilament protein, Light molecular weight neurofilament protein, NEFL, Neurofilament light polypeptide 68kDa, Neurofilament light polypeptide, Neurofilament protein, light chain, Neurofilament subunit NF-L, Neurofilament triplet L protein, NF-L, NF68, anti-NEFH antibody, anti-Neurofilament H antibody, anti-Neurofilament Heavy Polypeptide 2kDa antibody, anti-Neurofilament Triplet H Protein antibody, anti-NF-H antibody, anti-NF2. 68kDa neurofilament protein antibody, anti-Light molecular weight neurofilament protein antibody, anti-NEFL antibody, anti-Neurofilament light polypeptide 68kDa antibody, anti-Neurofilament light polypeptide antibody, anti-Neurofilament protein antibody, anti-light chain antibody, anti-Neurofilament subunit NF-L antibody, anti-Neurofilament triplet L protein antibody, anti-NF-L antibody, anti-NF68 antibody
Molecular Weight
200kDa & 68kDa
Chromosomal Location
22q12.2 (NEFH) & 8p21.2 (NEFL)
Curated Database and Bioinformatic Data
Gene SymbolNEFH & NEFL
Entrez Gene ID4744
Ensemble Gene IDENSG00000100285
RefSeq Protein Accession(s)XP_011528502, NP_066554
RefSeq mRNA Accession(s)NM_021076, XM_011530200
RefSeq Genomic Accession(s)NG_008404, NC_018933, NC_000022
UniProt ID(s)P12036
UniGene ID(s)P12036
HGNC ID(s)7737
Cosmic ID(s)NEFH
KEGG Gene ID(s)hsa:4744
PharmGKB ID(s)PA31540
General Description of Neurofilament.
This MAb reacts with a 200kDa, 68kDa protein, identified as heavy, light sub-units of neurofilaments (NF-H & NF-L). Neurofilaments make up the main structural elements of axons, dendrites, are found in neurons, peripheral nerves,, sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M), 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine,, endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas,, neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal, extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin,, oat cell carcinomas of the lung also express neurofilament.

Human, Mouse, Rat, Chicken, and Pig Anti-Neurofilament Antibody Product Attributes

Species: Human, Mouse, Rat, Chicken, and Pig
Tested Applications: Flow Cytometry, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.25-0.5ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Neurofilament Antibody Clone: RT-97 + NR-4
Clone RT-97 + NR-4 Host and Isotype: Mouse IgG
Anti-Human, Mouse, Rat, Chicken, and Pig Neurofilament Positive Control Sample: Cerebellum, Neuroblastoma
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Triton-X 100 insoluble proteins of rat brain (RT-97); Neurofilaments from porcine spinal cord (NR-4)
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Neurofilament Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-NEFH antibody staining is expected to be primarily localized to the centrosome, nuclear bodies and nucleus.

Limitations and Warranty

enQuire Bio's Neurofilament Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Neurofilament Antibody Clone RT-97 + NR-4