Formalin-fixed, paraffin-embedded human Cerebellum stained with Neurofilament Monoclonal Antibody (SPM145).
Formalin-fixed, paraffin-embedded human Cerebellum stained with Neurofilament Monoclonal Antibody (SPM145).

Anti-Neurofilament Antibody Clone SPM145

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: MSM2X-1201
Species: Human, Mouse, and Rat
Tested Applications: Flow Cytometry, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1
Mass Spec Validated?: Not MS Validated

Size
Tag Clear selection
Shipping Information
In Stock: 1-2 Day Lead Time.
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
SKU: MSM2X-1201-P1
Neurofilament General Information
Alternate Names
NEFH, Neurofilament H, Neurofilament Heavy Polypeptide 2kDa, Neurofilament Triplet H Protein, NF-H, NF2. 68kDa neurofilament protein, Light molecular weight neurofilament protein, NEFL, Neurofilament light polypeptide 68kDa, Neurofilament light polypeptide, Neurofilament protein, light chain, Neurofilament subunit NF-L, Neurofilament triplet L protein, NF-L, NF68, anti-NEFH antibody, anti-Neurofilament H antibody, anti-Neurofilament Heavy Polypeptide 2kDa antibody, anti-Neurofilament Triplet H Protein antibody, anti-NF-H antibody, anti-NF2. 68kDa neurofilament protein antibody, anti-Light molecular weight neurofilament protein antibody, anti-NEFL antibody, anti-Neurofilament light polypeptide 68kDa antibody, anti-Neurofilament light polypeptide antibody, anti-Neurofilament protein antibody, anti-light chain antibody, anti-Neurofilament subunit NF-L antibody, anti-Neurofilament triplet L protein antibody, anti-NF-L antibody, anti-NF68 antibody
Molecular Weight
200kDa & 68kDa
Chromosomal Location
22q12.2 (NEFH) & 8p21.2 (NEFL)
Curated Database and Bioinformatic Data
Gene SymbolNEFH & NEFL
Entrez Gene ID4744
Ensemble Gene IDENSG00000100285
RefSeq Protein Accession(s)XP_011528502, NP_066554
RefSeq mRNA Accession(s)NM_021076, XM_011530200
RefSeq Genomic Accession(s)NG_008404, NC_018933, NC_000022
UniProt ID(s)P12036
UniGene ID(s)P12036
HGNC ID(s)7737
Cosmic ID(s)NEFH
KEGG Gene ID(s)hsa:4744
PharmGKB ID(s)PA31540
General Description of Neurofilament.
This MAb reacts with a 200kDa, 68kDa protein, identified as heavy, light sub-units of neurofilaments (NF-H & NF-L). Neurofilaments make up the main structural elements of axons, dendrites, are found in neurons, peripheral nerves,, sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M), 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine,, endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas,, neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal, extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin,, oat cell carcinomas of the lung also express neurofilament.

Human, Mouse, and Rat Anti-Neurofilament Antibody Product Attributes

Species: Human, Mouse, and Rat
Tested Applications: Flow Cytometry, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.25-0.5ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Neurofilament Antibody Clone: SPM145
Clone SPM145 Host and Isotype: Mouse IgG1
Anti-Human, Mouse, and Rat Neurofilament Positive Control Sample: Cerebellum, Neuroblastoma
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Semi-purified human neurofilaments from spinal cord
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Neurofilament Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-NEFH antibody staining is expected to be primarily localized to the centrosome, nuclear bodies and nucleus.

Limitations and Warranty

enQuire Bio's Neurofilament Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

There are no reviews yet.

Be the first to review “Anti-Neurofilament Antibody Clone SPM145”

Your email address will not be published. Required fields are marked *

Share a Protocol (View Reviewed Protocols Below)

Protocol Title

Materials

Methods

Notes


  protocol submission gif

enQuire Bio, LLC   1825 Lawrence St, Ste 115   Denver, CO 80202
Phone: 1-855-344-1400 | enquire@enquirebio.com | Fax: 1-855-344-1400
Customer Service Available M-F 8AM-6PM MST or 24/7 Via Email
Scroll to top

We consume cookies ensure that we give you the best experience on our website. If you continue to use this site we will assume that you are happy with it. Dismiss

Anti-Neurofilament Antibody Clone SPM145https://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-MSM2X-1201-P1-anti-Neurofilament-antibody-300x225.jpeg