Formalin-fixed, paraffin-embedded Cerebellum stained with Neurofilament Monoclonal Antibody (SPM24).
Formalin-fixed, paraffin-embedded Cerebellum stained with Neurofilament Monoclonal Antibody (SPM24).

Anti-Neurofilament Antibody Clone SPM204

$ 488.00
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SKU: 4747-MSM1X
Species: Human, Rat, Pig, Cow, and Chicken
Tested Applications: Immunofluorescence, Flow Cytometry, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
4747-MSM1X-P1 Size: 100 ug, Tag: Unconjugated
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SKU: 4747-MSM1X-P1
Neurofilament General Information
Alternate Names
Neurofilament light polypeptide, NEFL
Molecular Weight
Chromosomal Location
Curated Database and Bioinformatic Data
Gene SymbolNEFL
Entrez Gene ID4747
Ensemble Gene IDENSG00000277586
RefSeq Protein Accession(s)NP_006149
RefSeq mRNA Accession(s)NM_006158
RefSeq Genomic Accession(s)NC_000008, NG_008492, NC_018919
UniProt ID(s)P07196
UniGene ID(s)P07196
HGNC ID(s)7739
Cosmic ID(s)NEFL
KEGG Gene ID(s)hsa:4747
PharmGKB ID(s)PA31542
General Description of Neurofilament.
This MAb reacts with a 68kDa protein, identified as light sub-unit of neurofilaments (NF-L). Neurofilaments make up the main structural elements of axons, dendrites, are found in neurons, peripheral nerves,, sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M), 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine,, endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas,, neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal, extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin,, oat cell carcinomas of the lung also express neurofilament.

Human, Rat, Pig, Cow, and Chicken Anti-Neurofilament Antibody Product Attributes

Species: Human, Rat, Pig, Cow, and Chicken
Tested Applications: Immunofluorescence, Flow Cytometry, Immunohistochemistry (IHC).
Application Notes: Immunofluorescence (1-2ug of antibody/ml), Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.25-0.5ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Neurofilament Antibody Clone: SPM204
Clone SPM204 Host and Isotype: Mouse IgG1
Anti-Human, Rat, Pig, Cow, and Chicken Neurofilament Positive Control Sample: Brain, Neuroblastoma
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Neurofilament purified from human brain
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Neurofilament Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-NEFL antibody staining is expected to be primarily localized to the intermediate filaments and nuclear interchromatin granular clusters. There is variability in either the signal strength or the localization of signal in intermediate filaments from cell to cell.

Observed Antibody Staining Data By Tissue Type:

Variations in Neurofilament antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in neuronal cells in the caudate nucleus, cerebral cortex. More moderate antibody staining intensity was present in neuronal cells in the caudate nucleus, cerebral cortex. Low, but measureable presence of Neurofilament could be seen in cells in the granular layer in cerebellum. We were unable to detect Neurofilament in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Limitations and Warranty

enQuire Bio's Neurofilament Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Neurofilament Antibody Clone SPM204