Immunohistochemical staining of Neuron Specific Enolase, Bovine  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

Neuron Specific Enolase, Bovine [Polyclonal] Antibody (cGMP).

$ 135.00$ 435.00
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Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Rabbit Undetermined

Product NumberDescriptionPrice
QIVD162-0.1ml Size: 0.1 ml, Format: Concentrate $ 135.00
QIVD162-0.5ml Size: 0.5 ml, Format: Concentrate $ 265.00
QIVD162-1ml Size: 1 ml, Format: Concentrate $ 435.00
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Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

Expiration Date:

Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

Manufacture Date:


Bioactivity (test results eg. IU/ml):

SKU: QIVD162-0.1ml
Neuron Specific Enolase, Bovine General Information
Alternate Names
Molecular Weight
47.3 kDa
Chromosomal Location
[chr: 5] [chr_start: 103538156] [chr_end: 103547189] [strand: -1]
Curated Database and Bioinformatic Data
Gene SymbolENO2
Entrez Gene ID526006
RefSeq Protein Accession(s)NP_001094595; XP_005207208
RefSeq mRNA Accession(s)NM_001101125; XM_005207151
RefSeq Genomic Accession(s)NC_037332
UniProt ID(s)A6QR19
KEGG Gene ID(s)bta:526006
General Description of Neuron Specific Enolase, Bovine .
This antibody reacts with human isoenzymes of neuron specific enolase containing γ subunits. This antibody gives a specific staining in neurons of the brain, in islets of Langerhans in pancreas and in peripheral nerves of the skin. No staining with other tissue types has been seen.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:25-1:50
Pretreatment Citrate Buffer pH 6.0
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Polyclonal
Anti-Neuron Specific Enolase, Bovine Antibody Clone: Polyclonal
Host and Isotype: Rabbit Undetermined
Recommended Positive Control Sample: Pancreas
Cellular Localization of Antibody Polyclonal Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: Neuron specific enolase (γ-γ isoenzyme) isolated from bovine brain.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

Neuron Specific Enolase, Bovine Information for Pathologists


Cytoplasmic enzyme expressed by neuroendocrine cells and tumors, including poorly differentiated neuroendocrine tumors, which usually are negative for other neuroendocrine markers. Despite its name, is often not specific for neuroendocrine cells / tumors. Enolases are widely distributed glycolytic enzymes that catalyze the interconversion of 2-phosphoglycerate and phosphophenolpyruvate. NSE refers to the gamma-gamma and alpha-gamma isoenzymes preferentially found in neurons and neuroendocrine cells. Microscopic (histologic) images

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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Neuron Specific Enolase, Bovine [Polyclonal] Antibody (cGMP).