Immunohistochemical staining of Neuron Specific Enolase  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

Neuron Specific Enolase [VI-H14] Antibody (cGMP).

$ 168.00$ 1,018.00
Please Select Product Options Below To View The Catalog Number.

SKU: QIVD168
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Mouse IgG1

Product NumberDescriptionPrice
QIVD168-0.1ml Size: 0.1 ml, Format: Concentrate $ 145.00
QIVD168-0.5ml Size: 0.5 ml, Format: Concentrate $ 555.00
QIVD168-1ml Size: 1 ml, Format: Concentrate $ 905.00
QIVD168-6ml Size: 6 ml, Format: Predilute $ 465.00
QIVD168-25ml Size: 25 ml, Format: Predilute $ 905.00
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Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
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SKU: QIVD168-0.1ml
Neuron Specific Enolase General Information
Alternate Names
Molecular Weight
47.3 kDa
Chromosomal Location
p13.31 [chr: 12] [chr_start: 6913745] [chr_end: 6923698] [strand: 1]
Curated Database and Bioinformatic Data
Gene SymbolENO2
Entrez Gene ID2026
RefSeq Protein Accession(s)NP_001966
RefSeq mRNA Accession(s)NM_001975
RefSeq Genomic Accession(s)NC_000012
UniProt ID(s)P09104
PharmGKB ID(s)PA27788
KEGG Gene ID(s)hsa:2026
General Description of Neuron Specific Enolase .
This antibody is specific to γ-γ enolase isozyme of 46 kDa. This antibody stains neurons, neuroendocrine cells, gangliomas, oat cell carcinomas, astrocytomas, paragangliomas, glioblastomas, pheochromocytomas, melanomas and schwannomas.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:25-1:50
Pretreatment Citrate Buffer pH 6.0
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-Neuron Specific Enolase Antibody Clone: VI-H14
Host and Isotype: Mouse IgG1
Recommended Positive Control Sample: Pancreas
Cellular Localization of Antibody VI-H14 Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: BALB/C mice were injected with γ-γ enolase from human brain.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

Neuron Specific Enolase Information for Pathologists

Summary:

Cytoplasmic enzyme expressed by neuroendocrine cells and tumors, including poorly differentiated neuroendocrine tumors, which usually are negative for other neuroendocrine markers. Despite its name, is often not specific for neuroendocrine cells / tumors. Enolases are widely distributed glycolytic enzymes that catalyze the interconversion of 2-phosphoglycerate and phosphophenolpyruvate. NSE refers to the gamma-gamma and alpha-gamma isoenzymes preferentially found in neurons and neuroendocrine cells. Microscopic (histologic) images

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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Neuron Specific Enolase [VI-H14] Antibody (cGMP).