Antibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
|Recommended Dilution (Conc)||1:25-1:50|
|Pretreatment||Citrate Buffer pH 6.0|
|Incubation Parameters||30 min at Room Temperature|
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Anti-Neuron Specific Enolase Antibody Clone: VI-H14
Host and Isotype: Mouse IgG1
Recommended Positive Control Sample: Pancreas
Cellular Localization of Antibody VI-H14 Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: BALB/C mice were injected with Î³-Î³ enolase from human brain.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
Neuron Specific Enolase Information for Pathologists
Summary:Cytoplasmic enzyme expressed by neuroendocrine cells and tumors, including poorly differentiated neuroendocrine tumors, which usually are negative for other neuroendocrine markers. Despite its name, is often not specific for neuroendocrine cells / tumors. Enolases are widely distributed glycolytic enzymes that catalyze the interconversion of 2-phosphoglycerate and phosphophenolpyruvate. NSE refers to the gamma-gamma and alpha-gamma isoenzymes preferentially found in neurons and neuroendocrine cells. Microscopic (histologic) images
Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
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