Formalin-fixed, paraffin-embedded human Melanoma stained with NGFR Monoclonal Antibody (NGFR5).
Formalin-fixed, paraffin-embedded human Melanoma stained with NGFR Monoclonal Antibody (NGFR5).

Anti-NGF-Receptor / CD271 Antibody Clone NGFR5

$ 298.00
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SKU: 4804-MSM1
Species: Human, Monkey, Baboon, Cat, Rabbit, Ferret, Mouse (-), and Rat (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
4804-MSM1-P1 Size: 100 ug, Tag: Unconjugated
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In Stock.
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SKU: 4804-MSM1-P1
NGF-Receptor / CD271 General Information
Alternate Names
low-affinity nerve growth factor receptor, nerve growth factor receptor, TNFR superfamily, member 16, LNGFR, p75 neurotrophin receptor, p75 NTR, p75 NGF receptor
Molecular Weight
75kDa
Chromosomal Location
17q21-22
Curated Database and Bioinformatic Data
Gene SymbolNGFR
Entrez Gene ID4804
Ensemble Gene IDENSG00000064300
RefSeq Protein Accession(s)NP_002498
RefSeq mRNA Accession(s)NM_002507
RefSeq Genomic Accession(s)NC_018928, NC_000017
UniProt ID(s)P08138
UniGene ID(s)P08138
HGNC ID(s)7809
Cosmic ID(s)NGFR
KEGG Gene ID(s)hsa:4804
PharmGKB ID(s)PA31615
General Description of NGF-Receptor / CD271.
It recognizes a glycoprotein of 75kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). Its epitope spans in aa 1-160 of extracellular domain of NGFR/NTR. NGF-receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain,, a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. NGF is important for the development, differentiation,, survival of variety of neuronal, non-neuronal cells. Its action is mediated by binding two distinct receptors, the high affinity p140, low affinity p75.

Human, Monkey, Baboon, Cat, Rabbit, Ferret, Mouse (-), and Rat (-) Anti-NGF-Receptor / CD271 Antibody Product Attributes

Species: Human, Monkey, Baboon, Cat, Rabbit, Ferret, Mouse (-), and Rat (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-NGF-Receptor / CD271 Antibody Clone: NGFR5
Clone NGFR5 Host and Isotype: Mouse IgG1 kappa
Anti-Human, Monkey, Baboon, Cat, Rabbit, Ferret, Mouse (-), and Rat (-) NGF-Receptor / CD271 Positive Control Sample: Neuronal axons, Schwann cells,, perineural cells of peripheral nerves, or tumors of nerve sheath differentiation e.g. Schwannoma, Neurofibroma. Soma, axons of sensory neurons,, ganglionic satellite cells. Melanomas.
Cellular Localization of Antibody Cell surface, cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: NGFR from A875 melanoma cells
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

NGF-Receptor / CD271 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-NGFR antibody staining is expected to be primarily localized to the nucleoplasm and plasma membrane.

Observed Antibody Staining Data By Tissue Type:

Variations in NGF-Receptor / CD271 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in peripheral nerve/ganglion in colon. More moderate antibody staining intensity was present in peripheral nerve/ganglion in colon. Low, but measureable presence of NGF-Receptor / CD271 could be seen inrespiratory epithelial cells in the bronchus, squamous epithelial cells in the cervix, uterine and esophagus, glandular cells in the gallbladder, respiratory epithelial cells in the nasopharynx, squamous epithelial cells in the oral mucosa, keratinocytes in skin and squamous epithelial cells in the tonsil. We were unable to detect NGF-Receptor / CD271 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of NGF-Receptor / CD271 expression as measured by anti-NGF-Receptor / CD271 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - + - - - - - - - - - - - - - -
NGFR Variability + ++ + + + ++ ++ + + ++ + + + + + ++ + + + ++

Limitations and Warranty

enQuire Bio's NGF-Receptor / CD271 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-NGF-Receptor / CD271 Antibody Clone NGFR5

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