Formalin-fixed paraffin-embedded human Pancreas stained with NKX2.2 Monoclonal Antibody (SPM564).
Formalin-fixed paraffin-embedded human Pancreas stained with NKX2.2 Monoclonal Antibody (SPM564).

Anti-NKX2.2 Antibody Clone SPM564

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: 4821-MSM1X
Species: Human, Mouse, Rat, and chicken
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2b kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
4821-MSM1X-P1 Size: 100 ug, Tag: Unconjugated
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In Stock.
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SKU: 4821-MSM1X-P1
NKX2.2 General Information
Alternate Names
Homeobox protein Nkx-2, NKX2-2
Molecular Weight
40-50kDa
Chromosomal Location
20p11.22
Curated Database and Bioinformatic Data
Gene SymbolNKX2-2
Entrez Gene ID4821
Ensemble Gene IDENSG00000125820
RefSeq Protein Accession(s)NP_002500, XP_006723629
RefSeq mRNA Accession(s)XM_006723566, NM_002509,
RefSeq Genomic Accession(s)NC_000020, NG_042186, NC_018931
UniProt ID(s)O95096
UniGene ID(s)O95096
HGNC ID(s)7835
Cosmic ID(s)NKX2-2
KEGG Gene ID(s)hsa:4821
PharmGKB ID(s)PA31642
General Description of NKX2.2.
Expression of NKX2.2 has been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors. More recently, NKX2.2 protein was identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma,, was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. It acts as a valuable marker for Ewing sarcoma, with a sensitivity of 93%, a specificity of 89%,, aids in the differential diagnosis of small round cell tumors.

Human, Mouse, Rat, and chicken Anti-NKX2.2 Antibody Product Attributes

Species: Human, Mouse, Rat, and chicken
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-NKX2.2 Antibody Clone: SPM564
Clone SPM564 Host and Isotype: Mouse IgG2b kappa
Anti-Human, Mouse, Rat, and chicken NKX2.2 Positive Control Sample: Pancreas or Ewing sarcoma
Cellular Localization of Antibody Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Human NKX2.2 recombinant protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

NKX2.2 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in NKX2.2 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of NKX2.2 could be seen in cells in the molecular layer in cerebellum, glandular cells in the duodenum and epididymis, neuronal cells in the hippocampus, cells in the tubules in kidney, exocrine glandular cells in the pancreas, islets of Langerhans in pancreas, glandular cells in the seminal vesicle, epidermal cells in the skin, glandular cells in the small intestine and stomach, cells in the seminiferous ducts in testis, Leydig cells in the testis, non-germinal center cells in the tonsil and squamous epithelial cells in the tonsil. We were unable to detect NKX2.2 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of NKX2.2 expression as measured by anti-NKX2.2 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity ++ + - + + + - - - - - - - ++ + - + - - -
NKX2-2 Variability ++ ++ + ++ ++ ++ ++ + + + ++ ++ ++ + ++ + ++ ++ + ++

Limitations and Warranty

enQuire Bio's NKX2.2 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-NKX2.2 Antibody Clone SPM564

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