Formalin-fixed, paraffin-embedded human Tonsil stained with NuMA Monoclonal Antibody (A73-B/D12)
Formalin-fixed, paraffin-embedded human Tonsil stained with NuMA Monoclonal Antibody (A73-B/D12)

Anti-Nuclear Mitotic Apparatus Protein Antibody Clone A73-B/D12

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: 4926-MSM1
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgM kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
4926-MSM1-P1 Size: 100 ug, Tag: Unconjugated
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SKU: 4926-MSM1-P1
Nuclear Mitotic Apparatus Protein General Information
Alternate Names
Nuclear mitotic apparatus protein 1, NUMA1
Molecular Weight
228kDa
Chromosomal Location
11q13
Curated Database and Bioinformatic Data
Gene SymbolNUMA1
Entrez Gene ID4926
Ensemble Gene IDENSG00000137497
RefSeq Protein Accession(s)XP_006718627, XP_011543356, XP_011543366, XP_016873319, XP_011543362, XP_011543363, XP_011543365, XP_011543361, XP_011543368, XP_011543359, XP_011543358, XP_011543364, NP_001273490, XP_011543357, XP_011543360, XP_011543367, XP_016873320, NP_006176
RefSeq mRNA Accession(s)XM_011545059, XM_011545066, XM_017017831, NM_001286561, XM_011545054, XM_011545060, XM_011545064, XM_011545062, XM_011545065, XM_017017830, NR_104476, XM_011545063, XM_011545055, XM_011545061, NM_006185, XM_011545057, XM_011545058, XM_006718564, XM_011545056
RefSeq Genomic Accession(s)NC_018922, NC_000011
UniProt ID(s)A0A024R5M9, Q4LE64, Q14980, Q3SYK8
UniGene ID(s)A0A024R5M9, Q4LE64, Q14980, Q3SYK8
HGNC ID(s)8059
Cosmic ID(s)NUMA1
KEGG Gene ID(s)hsa:4926
PharmGKB ID(s)PA31844
General Description of Nuclear Mitotic Apparatus Protein.
Recognizes a phosphorylated protein of 228kDa, identified as nuclear mitotic apparatus protein (NuMA). Its epitope is resistant to phosphatases. NuMA is intra-nuclear protein, present in nucleus during interphase. At the onset of mitosis, it redistributes from the nucleus to two centrosomal structures that later will become part of the mitotic spindle pole. After anaphase, the protein redistributes from the spindle polar region into reforming nucleus. NuMA is an essential protein during mitosis for the terminal phases of chromosome separation,/or nuclear reassembly. Recently a study shows that NuMA is cleaved to a 180 to 200kDa during apoptosis. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 has been detected in patients with acute promyelocytic leukemia.

Human Anti-Nuclear Mitotic Apparatus Protein Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Nuclear Mitotic Apparatus Protein Antibody Clone: A73-B/D12
Clone A73-B/D12 Host and Isotype: Mouse IgM kappa
Anti-Human Nuclear Mitotic Apparatus Protein Positive Control Sample: Exponentially growing any cultured human cells. Tonsil or lymph node.
Cellular Localization of Antibody Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Colon carcinoma 174T cells
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Nuclear Mitotic Apparatus Protein Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-NUMA1 antibody staining is expected to be primarily localized to the nucleoplasm.

Observed Antibody Staining Data By Tissue Type:

Variations in Nuclear Mitotic Apparatus Protein antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glandular cells in the adrenal gland and appendix, hematopoietic cells in the bone marrow, glandular cells in the breast, myoepithelial cells in the breast, respiratory epithelial cells in the bronchus, glial cells in the caudate nucleus, neuronal cells in the caudate nucleus, cells in the granular layer in cerebellum, cells in the molecular layer in cerebellum, Purkinje cells in the cerebellum, glial cells in the cerebral cortex, neuronal cells in the cerebral cortex, glandular cells in the cervix, uterine, squamous epithelial cells in the cervix, uterine, endothelial cells in the colon, glandular cells in the colon, peripheral nerve/ganglion in colon, glandular cells in the duodenum, cells in the endometrial stroma in endometrium, glandular cells in the endometrium and epididymis, squamous epithelial cells in the esophagus, glandular cells in the fallopian tube and gallbladder, myocytes in heart muscle, glial cells in the hippocampus, neuronal cells in the hippocampus, cells in the glomeruli in kidney, cells in the tubules in kidney, macrophages in lung, pneumocytes in lung, non-germinal center cells in the lymph node, respiratory epithelial cells in the nasopharynx, squamous epithelial cells in the oral mucosa, ovarian stroma cells in the ovary, exocrine glandular cells in the pancreas, islets of Langerhans in pancreas, glandular cells in the parathyroid gland, decidual cells in the placenta, trophoblastic cells in the placenta, glandular cells in the prostate, rectum, salivary gland and seminal vesicle, myocytes in skeletal muscle, fibroblasts in skin, keratinocytes in skin, Langerhans in skin, melanocytes in skin, epidermal cells in the skin, glandular cells in the small intestine, smooth muscle cells in the smooth muscle, adipocytes in mesenchymal tissue, chondrocytes in mesenchymal tissue, fibroblasts in mesenchymal tissue, peripheral nerve in mesenchymal tissue, cells in the red pulp in spleen, cells in the white pulp in spleen, glandular cells in the stomach, cells in the seminiferous ducts in testis, Leydig cells in the testis, glandular cells in the thyroid gland, non-germinal center cells in the tonsil, squamous epithelial cells in the tonsil, urothelial cells in the urinary bladder and squamous epithelial cells in the vagina. More moderate antibody staining intensity was present in glandular cells in the adrenal gland and appendix, hematopoietic cells in the bone marrow, glandular cells in the breast, myoepithelial cells in the breast, respiratory epithelial cells in the bronchus, glial cells in the caudate nucleus, neuronal cells in the caudate nucleus, cells in the granular layer in cerebellum, cells in the molecular layer in cerebellum, Purkinje cells in the cerebellum, glial cells in the cerebral cortex, neuronal cells in the cerebral cortex, glandular cells in the cervix, uterine, squamous epithelial cells in the cervix, uterine, endothelial cells in the colon, glandular cells in the colon, peripheral nerve/ganglion in colon, glandular cells in the duodenum, cells in the endometrial stroma in endometrium, glandular cells in the endometrium and epididymis, squamous epithelial cells in the esophagus, glandular cells in the fallopian tube and gallbladder, myocytes in heart muscle, glial cells in the hippocampus, neuronal cells in the hippocampus, cells in the glomeruli in kidney, cells in the tubules in kidney, macrophages in lung, pneumocytes in lung, non-germinal center cells in the lymph node, respiratory epithelial cells in the nasopharynx, squamous epithelial cells in the oral mucosa, ovarian stroma cells in the ovary, exocrine glandular cells in the pancreas, islets of Langerhans in pancreas, glandular cells in the parathyroid gland, decidual cells in the placenta, trophoblastic cells in the placenta, glandular cells in the prostate, rectum, salivary gland and seminal vesicle, myocytes in skeletal muscle, fibroblasts in skin, keratinocytes in skin, Langerhans in skin, melanocytes in skin, epidermal cells in the skin, glandular cells in the small intestine, smooth muscle cells in the smooth muscle, adipocytes in mesenchymal tissue, chondrocytes in mesenchymal tissue, fibroblasts in mesenchymal tissue, peripheral nerve in mesenchymal tissue, cells in the red pulp in spleen, cells in the white pulp in spleen, glandular cells in the stomach, cells in the seminiferous ducts in testis, Leydig cells in the testis, glandular cells in the thyroid gland, non-germinal center cells in the tonsil, squamous epithelial cells in the tonsil, urothelial cells in the urinary bladder and squamous epithelial cells in the vagina. Low, but measureable presence of Nuclear Mitotic Apparatus Protein could be seen inhepatocytes in liver. We were unable to detect Nuclear Mitotic Apparatus Protein in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Nuclear Mitotic Apparatus Protein expression as measured by anti-Nuclear Mitotic Apparatus Protein antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity +++ +++ ++ ++ +++ +++ +++ ++ +++ ++ +++ +++ +++ +++ ++ +++ ++ ++ +++ +++
NUMA1 Variability + ++ ++ ++ ++ + ++ ++ ++ ++ ++ + ++ + ++ ++ ++ ++ + ++

Limitations and Warranty

enQuire Bio's Nuclear Mitotic Apparatus Protein Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Nuclear Mitotic Apparatus Protein Antibody Clone A73-B/D12

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