Immunohistochemical staining of 43742 of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

OCT-4 [NRG1.1] Antibody (cGMP).

$ 158.00$ 748.00
Please Select Product Options Below To View The Catalog Number.

SKU: QIVD163
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Mouse IgG2b

Product NumberDescriptionPrice
QIVD163-0.1ml Size: 0.1 ml, Format: Concentrate $ 158.00
QIVD163-0.5ml Size: 0.5 ml, Format: Concentrate $ 468.00
QIVD163-1ml Size: 1 ml, Format: Concentrate $ 748.00
QIVD163-6ml Size: 6 ml, Format: Predilute $ 288.00
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Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

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Reconsitution Instructions (Leave Blank if Liquid):

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SKU: QIVD163-0.1ml
OCT-4 General Information
Alternate Names
Molecular Weight
38.6 kDa
Chromosomal Location
[chr: CHR_HSCHR6_MHC_QBL_CTG1] [chr_start: 31156777] [chr_end: 31163150] [strand: -1]; p21.33 [chr: 6] [chr_start: 31164337] [chr_end: 31180731] [strand: -1]; [chr: CHR_HSCHR6_MHC_DBB_CTG1] [chr_start: 31158060] [chr_end: 31164434] [strand: -1]; [chr: CHR_HSCHR6_MHC_COX_CTG1] [chr_start: 31156880] [chr_end: 31163254] [strand: -1]; [chr: CHR_HSCHR6_MHC_MANN_CTG1] [chr_start: 31209245] [chr_end: 31215620] [strand: -1]; [chr: CHR_HSCHR6_MHC_MCF_CTG1] [chr_start: 31242859] [chr_end: 31249234] [strand: -1]; [chr: CHR_HSCHR6_MHC_SSTO_CTG1] [chr_start: 31159218] [chr_end: 31165590] [strand: -1]
Curated Database and Bioinformatic Data
Gene SymbolPOU5F1
Entrez Gene ID5460
RefSeq Protein Accession(s)NP_002692; NP_001272916
RefSeq mRNA Accession(s)NM_001173531; NM_001285987; NM_001285986; NM_203289; NM_002701
RefSeq Genomic Accession(s)NT_167248; NT_167249; NT_113891; NT_167245; NT_167246; NC_000006; NT_167247
UniProt ID(s)Q01860
PharmGKB ID(s)PA33545
KEGG Gene ID(s)hsa:5460
General Description of OCT-4 .
OCT-4 (also known as POU5F1) is a transcription factor that has been recognized as fundamental in the maintenance of pluripotency in embryonic stem cells and primordial germ cells. It has been proposed as a useful marker for germ cell tumors (GCT) that exhibit features of pluripotentiality (seminoma/dysgerminoma, germinoma and embryonal carcinoma. OCT-4 immunostaining has been shown to be sensitive and specific for GCT, whether in primary gonadal or extragonadal sites or in metastatic lesions.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:25-1:50
Pretreatment Citrate Buffer pH 6.0
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-4-Oct Antibody Clone: NRG1.1
Host and Isotype: Mouse IgG2b
Recommended Positive Control Sample: Seminoma
Cellular Localization of Antibody NRG1.1 Staining: Nuclear
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: BALB/C mice injected with synthetic peptide of human OCT-4 origin.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

4-Oct Information for Pathologists

Summary:

OCT-4 (also Octamer-binding transcription factor 4) is an 18 kDa transription factor encoded by POU5F1. It is considered the master gene in pluripotency and embryonic stem cell regulation. It regulates self-renewal and differentiation of embryonic stem cells, include germ cells.

Common Uses By Pathologists:

OCT-4 is a Nuclear stain. It is highly sensitive and specific for primary intracranial germinomas which may be benign or malignant. Positive staining for OCT-4 is generally related to poor prognostis in esophageal squamous cell carcinoma and possibly in non-small cell lung cancer (NSCLC).

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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OCT-4 [NRG1.1] Antibody (cGMP).