Immunohistochemical staining of 43742 of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

OCT-4 [EP143] Antibody (cGMP).

$ 175.00$ 925.00
Please Select Product Options Below To View The Catalog Number.

SKU: QIVD164
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Rabbit IgG

Product NumberDescriptionPrice
QIVD164-0.1ml Size: 0.1 ml, Format: Concentrate $ 175.00
QIVD164-0.5ml Size: 0.5 ml, Format: Concentrate $ 585.00
QIVD164-1ml Size: 1 ml, Format: Concentrate $ 925.00
QIVD164-6ml Size: 6 ml, Format: Predilute $ 405.00
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Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

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SKU: QIVD164-0.1ml
OCT-4 General Information
Alternate Names
Molecular Weight
38.6 kDa
Chromosomal Location
[chr: CHR_HSCHR6_MHC_QBL_CTG1] [chr_start: 31156777] [chr_end: 31163150] [strand: -1]; p21.33 [chr: 6] [chr_start: 31164337] [chr_end: 31180731] [strand: -1]; [chr: CHR_HSCHR6_MHC_DBB_CTG1] [chr_start: 31158060] [chr_end: 31164434] [strand: -1]; [chr: CHR_HSCHR6_MHC_COX_CTG1] [chr_start: 31156880] [chr_end: 31163254] [strand: -1]; [chr: CHR_HSCHR6_MHC_MANN_CTG1] [chr_start: 31209245] [chr_end: 31215620] [strand: -1]; [chr: CHR_HSCHR6_MHC_MCF_CTG1] [chr_start: 31242859] [chr_end: 31249234] [strand: -1]; [chr: CHR_HSCHR6_MHC_SSTO_CTG1] [chr_start: 31159218] [chr_end: 31165590] [strand: -1]
Curated Database and Bioinformatic Data
Gene SymbolPOU5F1
Entrez Gene ID5460
RefSeq Protein Accession(s)NP_002692; NP_001272916
RefSeq mRNA Accession(s)NM_001173531; NM_001285987; NM_001285986; NM_203289; NM_002701
RefSeq Genomic Accession(s)NT_167248; NT_167249; NT_113891; NT_167245; NT_167246; NC_000006; NT_167247
UniProt ID(s)Q01860
PharmGKB ID(s)PA33545
KEGG Gene ID(s)hsa:5460
General Description of OCT-4 .
OCT-4 (octamer-binding transcription factor 4) also known as POU5F1 is a homeodomain transcription factor. It is involved in self-renewal of undifferentiated embryonic stem cells. It is frequently used as a marker for undifferentiated cells. OCT-4 expression must be closely regulated; too much or too little will cause differentiation of the cells. OCT-4 is one of the transcription factors used to create induced pluripotent stem cells. OCT-4 is sensitive and specific marker for germ cell tumors like seminomas, gonadoblastoma, germinoma and embryonal carcinomas but not for differentiated cells of nonseminomas, i.e. teratomas, yolk sac tumors and choriocarcinomas. OCT-4 can detect both primary and metastatic germ cell tumors.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:50-1:100
Pretreatment Citrate Buffer pH 6.0
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-4-Oct Antibody Clone: EP143
Host and Isotype: Rabbit IgG
Recommended Positive Control Sample: Seminoma for abnormal tissue
Cellular Localization of Antibody EP143 Staining: Nuclear
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: A synthetic peptide corresponding to residues of human OCT-4 protein
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

4-Oct Information for Pathologists

Summary:

OCT-4 (also Octamer-binding transcription factor 4) is an 18 kDa transription factor encoded by POU5F1. It is considered the master gene in pluripotency and embryonic stem cell regulation. It regulates self-renewal and differentiation of embryonic stem cells, include germ cells.

Common Uses By Pathologists:

OCT-4 is a Nuclear stain. It is highly sensitive and specific for primary intracranial germinomas which may be benign or malignant. Positive staining for OCT-4 is generally related to poor prognostis in esophageal squamous cell carcinoma and possibly in non-small cell lung cancer (NSCLC).

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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OCT-4 [EP143] Antibody (cGMP).