FFPE human prostate carcinoma sections stained with 100 ul anti-Ornithine Decarboxylase-1 (clone ODC1/485) at 1:50. HIER epitope retrieval prior to staining was performed in 10mM Citrate, pH 6.0.
FFPE human prostate carcinoma sections stained with 100 ul anti-Ornithine Decarboxylase-1 (clone ODC1/485) at 1:50. HIER epitope retrieval prior to staining was performed in 10mM Citrate, pH 6.0.Formalin-fixed, paraffin embedded human testicular carcinoma sections stained with 100 ul anti-Ornithine Decarboxylase-1 (clone ODC1/485) at 1:200. HIER epitope retrieval prior to staining was performed in 10mM Citrate, pH 6.0.Formalin-fixed, paraffin embedded rat pancreas sections stained with 100 ul anti-Ornithine Decarboxylase-1 (clone ODC1/485) at 1:400. HIER epitope retrieval prior to staining was performed in 10mM Citrate, pH 6.0.Flow Cytometry of human ODC1 on PC3 Cells. Black: Cells alone; Grey: Isotype Control; Green: Alexa Fluor® 488-labeled ODC1 Monoclonal Antibody (ODC1/485).

Anti-Ornithine Decarboxylase-1 Antibody Clone ODC1/485

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: 4953-MSM1
Species: Human and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
4953-MSM1-P1 Size: 100 ug, Tag: Unconjugated
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SKU: 4953-MSM1-P1
Ornithine Decarboxylase-1 General Information
Alternate Names
Ornithine decarboxylase, ODC1
Molecular Weight
53kDa
Chromosomal Location
2p25.1
Curated Database and Bioinformatic Data
Gene SymbolODC1
Entrez Gene ID4953
Ensemble Gene IDENSG00000115758
RefSeq Protein Accession(s)NP_001274117, NP_001274118, NP_001274119, NP_002530
RefSeq mRNA Accession(s)NM_001287189, NM_001287188, NM_002539 NM_001287190
RefSeq Genomic Accession(s)NC_000002, NC_018913, NG_012105
UniProt ID(s)B4DXF8, P11926
UniGene ID(s)B4DXF8, P11926
HGNC ID(s)8109
Cosmic ID(s)ODC1
KEGG Gene ID(s)hsa:4953
PharmGKB ID(s)PA31897
General Description of Ornithine Decarboxylase-1.
Recognizes a 53kDa protein, identified as the Ornithine Decarboxylase (ODC-1). ODC is the initial, rate-limiting enzyme in the biosynthetic pathway of polyamines, is involved in the conversion of ornithine to putrescine. The biological activity of ODC-1 is rapidly induced in response to virtually all agents known to promote cell proliferation including hormones, drugs, growth factors, mitogens,, tumor promoters. Reportedly, ODC mRNA levels are elevated in lung carcinomas as well as in colon adenomas, carcinomas. ODC activity in colorectal carcinomas is greater than those in adenomas, normal mucosa.

Human and Rat Anti-Ornithine Decarboxylase-1 Antibody Product Attributes

Species: Human and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.25-0.5ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Ornithine Decarboxylase-1 Antibody Clone: ODC1/485
Clone ODC1/485 Host and Isotype: Mouse IgG1 kappa
Anti-Human and Rat Ornithine Decarboxylase-1 Positive Control Sample: PC3 cells. Placenta, Prostate or Testicular carcinoma.
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human ODC-1 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Ornithine Decarboxylase-1 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-ODC1 antibody staining is expected to be primarily localized to the cytosol and plasma membrane.

Observed Antibody Staining Data By Tissue Type:

Variations in Ornithine Decarboxylase-1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in hepatocytes in liver, exocrine glandular cells in the pancreas, decidual cells in the placenta, glandular cells in the salivary gland and small intestine and Leydig cells in the testis. More moderate antibody staining intensity was present in hepatocytes in liver, exocrine glandular cells in the pancreas, decidual cells in the placenta, glandular cells in the salivary gland and small intestine and Leydig cells in the testis. Low, but measureable presence of Ornithine Decarboxylase-1 could be seen inglandular cells in the appendix, hematopoietic cells in the bone marrow, adipocytes in breast, glandular cells in the breast, respiratory epithelial cells in the bronchus, glial cells in the caudate nucleus, neuronal cells in the caudate nucleus, cells in the granular layer in cerebellum, cells in the molecular layer in cerebellum, Purkinje cells in the cerebellum, endothelial cells in the cerebral cortex, glial cells in the cerebral cortex, neuronal cells in the cerebral cortex, neuropil in cerebral cortex, endothelial cells in the colon, cells in the endometrial stroma in endometrium, squamous epithelial cells in the esophagus, glial cells in the hippocampus, neuronal cells in the hippocampus, pneumocytes in lung, non-germinal center cells in the lymph node, squamous epithelial cells in the oral mucosa, glandular cells in the parathyroid gland and rectum, melanocytes in skin, epidermal cells in the skin, adipocytes in mesenchymal tissue, chondrocytes in mesenchymal tissue, fibroblasts in mesenchymal tissue, peripheral nerve in mesenchymal tissue, cells in the seminiferous ducts in testis, germinal center cells in the tonsil, non-germinal center cells in the tonsil, urothelial cells in the urinary bladder and squamous epithelial cells in the vagina. We were unable to detect Ornithine Decarboxylase-1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Ornithine Decarboxylase-1 expression as measured by anti-Ornithine Decarboxylase-1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity ++ - ++ ++ ++ - - ++ + - - ++ + +++ + - + - + -
ODC1 Variability ++ ++ ++ ++ + + ++ ++ ++ + ++ ++ ++ + ++ ++ ++ + + ++

Limitations and Warranty

enQuire Bio's Ornithine Decarboxylase-1 Anti-Human, Rat Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Ornithine Decarboxylase-1 Antibody Clone ODC1/485

https://enquirebio.com/wp-content/uploads/2019/01/Ornithine Decarboxylase-1 IHC Prostate Carcinoma (ODC1 485).jpghttps://enquirebio.com/wp-content/uploads/2019/01/ODC1 IHC human Testicular Carcinoma (ODC1 485)-300x225.jpghttps://enquirebio.com/wp-content/uploads/2019/01/Ornithine Decarboxylase-1 IHC Rat Pancreas (ODC1 485)-300x225.jpghttps://enquirebio.com/wp-content/uploads/2019/01/LNCap ODC1 immunofluorescence-300x189.jpghttps://enquirebio.com/wp-content/uploads/2019/01/ODC1 WB in human placenta-300x168.jpghttps://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-4953-MSM1-P1-anti-Ornithine-Decarboxylase-1-antibody-300x224.jpeg