Immunohistochemical staining of p120 Catenin  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

p120 Catenin [EP66] Antibody (cGMP).

$ 135.00$ 865.00
Please Select Product Options Below To View The Catalog Number.

SKU: QIVD165
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Rabbit Rabbit IgG

Product NumberDescriptionPrice
QIVD165-0.1ml Size: 0.1 ml, Format: Concentrate $ 135.00
QIVD165-0.5ml Size: 0.5 ml, Format: Concentrate $ 545.00
QIVD165-1ml Size: 1 ml, Format: Concentrate $ 865.00
QIVD165-6ml Size: 6 ml, Format: Predilute $ 225.00
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SKU: QIVD165-0.1ml
p120 Catenin General Information
Alternate Names
Molecular Weight
108.2 kDa
Chromosomal Location
q12.1 [chr: 11] [chr_start: 57753243] [chr_end: 57819546] [strand: 1]
Curated Database and Bioinformatic Data
Gene SymbolCTNND1
Entrez Gene ID1500
RefSeq Protein Accession(s)NP_001078936; NP_001193818; NP_001322; NP_001078934; NP_001193819; NP_001078935; NP_001078928; NP_001193812; NP_001193816; NP_001193817; NP_001078932; NP_001193814; NP_001078927; NP_001078930; NP_001078937; NP_001078938; NP_001193815; NP_001078929; NP_001078931; NP_001078933; NP_001193813; NP_001193820
RefSeq mRNA Accession(s)NM_001085466; NM_001206888; NM_001206889; NM_001085459; NM_001206885; NM_001206886; NM_001206887; NM_001085465; NM_001206890; NM_001085458; NM_001085467; NM_001206883; NM_001331; NM_001085460; NM_001085461; NM_001085463; NM_001085468; NM_001085469; NM_001206884; NM_001085462; NM_001085464; NM_001206891
RefSeq Genomic Accession(s)NG_029078; NC_000011
UniProt ID(s)O60716
PharmGKB ID(s)PA27016
KEGG Gene ID(s)hsa:1500
Associated Diseases (KEGG IDs)Blepharocheilodontic syndrome 2 (BCDS2) [MIM:617681]: A form of blepharocheilodontic syndrome, a rare autosomal dominant disorder. It is characterized by lower eyelid ectropion, upper eyelid distichiasis, euryblepharon, bilateral cleft lip and palate, and features of ectodermal dysplasia, including hair anomalies, conical teeth and tooth agenesis. An additional rare manifestation is imperforate anus. There is considerable phenotypic variability among affected individuals. {ECO:0000269|PubMed:28301459}. The disease is caused by mutations affecting the gene represented in this entry.
General Description of p120 Catenin .
Cytoplasmic accumulation of p120 Catenin has been observed in lung cancer, pancreatic cancer, gastric cancer and colon cancers and is associated with poor progress in colon cancer patients. In breast lobular neoplasia, anti p120 Catenin shows a diffuse cytoplasmic immunostaining pattern,while breast ductal neoplasia retains the membrane immunostaining pattern. p120 Catenin antibody is useful in differentiation of lobular carcinoma from ductal carcinoma of the breast and in identifying early lesions of lobular neoplasia.rn

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:50-1:100
Pretreatment Citrate Buffer pH 6.0
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-p120 Catenin Antibody Clone: EP66
Host and Isotype: Rabbit Rabbit IgG
Recommended Positive Control Sample: Breast Large Intestine Breast Lobular Carcinoma
Cellular Localization of Antibody EP66 Staining: Membrane/Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: A synthetic peptide corresponding to residues in human p120 Catenin protein
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

p120 Catenin Information for Pathologists

Summary:

p120 catenin is one of the transmembrane components of epithelial adherens junction proteins (J Cell Sci 2001;114:503), which includes p120 catenin and E-cadherin (Prog Mol Biol Transl Sci 2013;116:71). In the cadherin - catenin complex, which is essential for the formation of intercellular tight junctions, p120 catenin attaches to E-cadherin on the internal aspect of the cell membrane. p120 is composed of 2 domains, an external calcium dependent domain and an internal domain to which p120 catenin is bound (Oncogene 1996;13:1919) in a juxtamembranous location (J Cell Biol 1998;141:779). p120 is involved in promotion of cell junction formation, cell motility and E-cadherin trafficking and turnover. Binding of p120 to E-cadherin stabilizes the complex and increases the half-life of E-cadherin.

Common Uses By Pathologists:

Differentiation between ductal and lobular carcinoma / in situ lesions. p120 shows a membranous pattern of staining in ductal carcinoma and in situ lesions. Lack of E-cadherin staining and strong p120 cytoplasmic staining is feature of the spectrum of lobular lesions (atypical lobular hyperplasia (ALH), lobular carcinoma in situ (LCIS), lobular carcinoma). Positive cytoplasmic staining for lobular carcinoma is much easier to interpret than E-cadherin negative staining. E-cadherin and p120 catenin cocktail improve the diagnosis of in situ lesions (Int J Clin Exp Pathol 2014;7:2551).

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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p120 Catenin [EP66] Antibody (cGMP).