Antibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
|Recommended Dilution (Conc)||1:50-1:100|
|Pretreatment||Citrate Buffer pH 6.0|
|Incubation Parameters||30 min at Room Temperature|
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Anti-p120 Catenin Antibody Clone: EP66
Host and Isotype: Rabbit Rabbit IgG
Recommended Positive Control Sample: Breast Large Intestine Breast Lobular Carcinoma
Cellular Localization of Antibody EP66 Staining: Membrane/Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: A synthetic peptide corresponding to residues in human p120 Catenin protein
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
p120 Catenin Information for Pathologists
Summary:p120 catenin is one of the transmembrane components of epithelial adherens junction proteins (J Cell Sci 2001;114:503), which includes p120 catenin and E-cadherin (Prog Mol Biol Transl Sci 2013;116:71). In the cadherin - catenin complex, which is essential for the formation of intercellular tight junctions, p120 catenin attaches to E-cadherin on the internal aspect of the cell membrane. p120 is composed of 2 domains, an external calcium dependent domain and an internal domain to which p120 catenin is bound (Oncogene 1996;13:1919) in a juxtamembranous location (J Cell Biol 1998;141:779). p120 is involved in promotion of cell junction formation, cell motility and E-cadherin trafficking and turnover. Binding of p120 to E-cadherin stabilizes the complex and increases the half-life of E-cadherin.
Common Uses By Pathologists:Differentiation between ductal and lobular carcinoma / in situ lesions. p120 shows a membranous pattern of staining in ductal carcinoma and in situ lesions. Lack of E-cadherin staining and strong p120 cytoplasmic staining is feature of the spectrum of lobular lesions (atypical lobular hyperplasia (ALH), lobular carcinoma in situ (LCIS), lobular carcinoma). Positive cytoplasmic staining for lobular carcinoma is much easier to interpret than E-cadherin negative staining. E-cadherin and p120 catenin cocktail improve the diagnosis of in situ lesions (Int J Clin Exp Pathol 2014;7:2551).
Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
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