Anti-p21WAF1Antibody Validation Data
Analysis of Mass Spec data (dashed-line) of fractions stained with p21WAF1 MS-QAVA™ monoclonal antibody [Clone: CIP1/823] (solid-line), reveals that less than 14.4% of signal is attributable to non-specific binding of anti-p21WAF1 [Clone CIP1/823] to targets other than CDKN1A protein. Even frequently cited antibodies have much greater non-specific interactions, averaging over 30%. Data in image is from analysis in A431, RT4 and MCF7 cells.

Anti-p21WAF1 Antibody Clone CIP1/823

$ 329.00
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SKU: 1026-MSM3
Species: Human, Mouse (-), and Rat (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2a kappa
Mass Spec Validated?: MS-QAVA™ Validated

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Datasheets and Documentation
Product Datasheet
SKU: 1026-MSM3-P1
p21WAF1 General Information
Alternate Names
cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, p21Cip1, p21 Cip1, p21Waf1, p21 Waf1
Molecular Weight
21kDa
Chromosomal Location
6p21.31
Curated Database and Bioinformatic Data
Gene SymbolCDKN1A
Entrez Gene ID1026
Ensemble Gene IDENSG00000124762
RefSeq Protein Accession(s)NP_001207706, NP_000380, NP_001207707, NP_001278478, NP_510867
RefSeq mRNA Accession(s)NM_001291549, NM_001220778, NM_000389, NM_078467, NM_001220777
RefSeq Genomic Accession(s)NG_009364, NC_000006, NC_018917
UniProt ID(s)A0A024RCX5, P38936
UniGene ID(s)A0A024RCX5, P38936
HGNC ID(s)1784
Cosmic ID(s)CDKN1A
KEGG Gene ID(s)hsa:1026
PharmGKB ID(s)PA104
General Description of p21WAF1.
This MAb recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This MAb is highly specific to p21, shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk s, a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase, is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma).

Human, Mouse (-), and Rat (-) Anti-p21WAF1 Antibody Product Attributes

Species: Human, Mouse (-), and Rat (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC). Additionally, this antibody has been validated by MS-QAVA™. Specificity has been quantitatively determined via Mass Spec.
Clonality: Monoclonal
Anti-p21WAF1 Antibody Clone: CIP1/823
Clone CIP1/823 Host and Isotype: Mouse IgG2a kappa
Anti-Human, Mouse (-), and Rat (-) p21WAF1 Positive Control Sample: HeLa Cells. Skin, colon, or breast carcinoma.
Cellular Localization of Antibody Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant full-length human CDKN1A protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

p21WAF1 Previously Observed Antibody Staining Patterns

Limitations and Warranty

This product is for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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